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High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes

  1. Author:
    Edgar, R.
    McKinstry, M.
    Hwang, J.
    Oppenheim, A. B.
    Fekete, R. A.
    Giulian, G.
    Merril, C.
    Nagashima, K.
    Adhya, S.

  2. Author Address

    NCI, Mol Biol Lab, Bethesda, MD 20892 USA. NIH, Bethesda, MD 20892 USA. Natl Inst Stand & Technol, Opt Technol Div, Phys Lab, Gaithersburg, MD 20899 USA. Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Mol Genet & Biotechnol, IL-91120 Jerusalem, Israel. Ambion Inc, Austin, TX 78744 USA. NCI, SAIC Frederick, Frederick, MD 21702 USA.;Adhya, S, NCI, Mol Biol Lab, 37 Convent Dr,Bldg 37,Room 5138, Bethesda, MD 20892 USA.;sadhya@helix.nih.gov
    1. Year: 2006
    2. Date: Mar
  2. Journal: Proceedings of the National Academy of Sciences of the United States of America
    1. 103
    2. 13
    3. Pages: 4841-4845
  4. Type of Article: Article
  5. ISSN: 0027-8424
  1. Abstract:

    With current concerns of antibiotic-resistant bacteria and biodefense, it has become important to rapidly identify infectious bacteria. Traditional technologies involving isolation and amplification of the pathogenic bacteria are time-consuming. We report a rapid and simple method that combines in vivo biotinylation of engineered host-specific bacteriophage and conjugation of the phage to streptavidin-coated quantum dots. The method provides specific detection of as few as 10 bacterial cells per milliliter in experimental samples, with an approximate to 100-fold amplification of the signal over background in 1 In. We believe that the method can be applied to any bacteria susceptible to specific phages and would be particularly useful for detection of bacterial strains that are slow growing, e.g., Mycobacterium, or are highly infectious, e.g., Bacillus anthracis. The potential for simultaneous detection of different bacterial species in a single sample and applications in the study of phage biology are discussed.

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External Sources

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  2. DOI: 10.1073/pnas.0601211103
  3. View record in Web of Science®
  4. WOS: 000236472500012

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