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Panmicrobial oligonucleotide array for diagnosis of infectious diseases

  1. Author:
    Palacios, G.
    Quan, P. L.
    Jabado, O. J.
    Conlan, S.
    Hirschberg, D. L.
    Liu, Y.
    Zhai, J.
    Renwick, N.
    Hui, J.
    Hegyi, H.
    Grolla, A.
    Strong, J. E.
    Towner, J. S.
    Geisbert, T. W.
    Jahrling, P. B.
    Buechen-Osmond, C.
    Ellerbrok, H.
    Sanchez-Seco, M. P.
    Lussier, Y.
    Formenty, P.
    Nichol, S. T.
    Feldmann, H.
    Briese, T.
    Lipkin, W. I.

  2. Author Address

    Columbia Univ, Jerome L & Dawn Greene Infect Dis Lab, Mailman Sch Publ Hlth, New York, NY 10032 USA. Stanford Univ, Stanford, CA 94305 USA. Univ Chicago, Chicago, IL 60637 USA. Inst Enzymol, Budapest, Hungary. Publ Hlth Agcy Canada, Winnipeg, MB, Canada. Ctr Dis Control & Prevent, Atlanta, GA USA. USA, Med Res Inst Infect Dis, Ft Detrick, MD 21702 USA. Natl Inst Hlth, Integrated Res Facil, Ft Detrick, MD 21702 USA. Robert Koch Inst, D-1000 Berlin, Germany. Inst Salud Carlos III, Madrid, Spain. World Hlth Org, Geneva, Switzerland. Univ Manitoba, Winnipeg, MB, Canada.;Lipkin, WI, Columbia Univ, Jerome L & Dawn Greene Infect Dis Lab, Mailman Sch Publ Hlth, 722 W 168th St,Rm 1801, New York, NY 10032 USA.;wil2001@columbia.edu
    1. Year: 2007
    2. Date: Jan
  2. Journal: Emerging Infectious Diseases
    1. 13
    2. 1
    3. Pages: 73-81
  4. Type of Article: Article
  5. ISSN: 1080-6040
  1. Abstract:

    To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.

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External Sources

  1. View record in Web of Science®
  2. WOS: 000243692500011

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