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Complete inactivation of Venezuelan equine encephalitis virus by 1,5-iodonaphthylazide

  1. Author:
    Sharma, A.
    Raviv, Y.
    Puri, A.
    Viard, M.
    Blumenthal, R.
    Maheshwari, R. K.

  2. Author Address

    Uniformed Serv Univ Hlth Sci, Ctr Combat Casualty & Life Sustainment Res, Dept Pathol, Bethesda, MD 20814 USA. Birla Inst Technol & Sci, Pilani, Rajasthan, India. NCI, CCR Nanobiol Program, Ctr Canc Res, Frederick, MD 21701 USA. Basic Res Program SAIC, Frederick, MD USA.;Maheshwari, RK, Uniformed Serv Univ Hlth Sci, Ctr Combat Casualty & Life Sustainment Res, Dept Pathol, 4301 Jones Bridge Rd, Bethesda, MD 20814 USA.;rmaheshwari@usuhs.mil
    1. Year: 2007
    2. Date: Jun
  2. Journal: Biochemical and Biophysical Research Communications
    1. 358
    2. 2
    3. Pages: 392-398
  4. Type of Article: Article
  5. ISSN: 0006-291X
  1. Abstract:

    Hydrophobic alkylating compounds like 1,5-iodonaphthylazide (INA) partitions into biological membranes and accumulates selectively into the hydrophobic domain of the lipid bilayer. Upon irradiation with far UV light, INA binds selectively to transmembrane proteins in the viral envelope and renders them inactive. Such inactivation does not alter the ectodomains of the membrane proteins thus preserving the structural and conformational integrity of immunogens on the surface of the virus. In this study, we have used INA to inactivate Venezuelan equine encephalitis virus (VEEV). Treatment of VEEV with INA followed by irradiation with UV light resulted in complete inactivation of the virus. Immuno-fluorescence for VEEV and virus titration showed no virus replication in-vitro. Complete loss of infectivity was also achieved in mice infected with INA treated plus irradiated preparations of VEEV. No change in the structural integrity of VEEV particles were observed after treatment with INA plus irradiation as assessed by electron microscopy. This data suggest that such inactivation strategies can be used for developing vaccine candidates for VEEV and other enveloped viruses. Published by Elsevier Inc.

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External Sources

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  2. DOI: 10.1016/j.bbrc.2007.04.115
  3. View record in Web of Science®
  4. WOS: 000246927300003

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