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Rtf1-mediated eukaryotic site-specific replication termination

  1. Author:
    Eydmann, T.
    Sommariva, E.
    Inagawa, T.
    Mian, S.
    Klar, A.
    Dalgaard, J. Z.

  2. Author Address

    Eydmann, T.; Sommariva, E.; Inagawa, T.; Dalgaard, J. Z.] Marie Curie Res Inst, Oxted RH8 0TL, Surrey, England. [Mian, S.] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA. [Klar, A. J. S.] NCI, Gene Regulat & Chromosome Biol Lab, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Genetics
    1. 180
    2. 1
    3. Pages: 27-39
  4. Type of Article: Article
  1. Abstract:

    The molecular mechanisms mediating eukaryotic replication termination and pausing remain largely unknown. Here we present the molecular characterization of Rtf1 that mediates site-specific replication termination at the polar Schizosaccharomyces pombe barrier RTS1. We show that Rtf1 possesses two chimeric myb/SANT domains: one is able to interact with the repeated motifs encoded by the RTS1 element as well as the elements enhancer region, while the other shows only a weak DNT binding activity. In addition we show that the C-terminal tail of Rtf1 mediates self-interaction, and deletion of this tail has a dominant phenotype. Finally, we identify a point imitation in Rtf1 domain I that converts the RTS1 element into a replication barrier of the opposite polarity. Together our data establish that multiple protein DNA and protein-protein interactions between Rtf1 molecules and both the repeated motifs and the enhancer region of RTS1 are required for site-specific termination at the RTS1 element.

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External Sources

  1. PMID: 18723894

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