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Structure-activity relationship studies for the peptide portion of the bladder epithelial cell antiproliferative factor from interstitial cystitis patients

  1. Author:
    Kaczmarek, P.
    Keay, S. K.
    Tocci, G. M.
    Koch, K. R.
    Zhang, C. O.
    Barchi, J. J.
    Grkovic, D.
    Guo, L.
    Michejda, C. J.

  2. Author Address

    Kaczmarek, Piotr, Tocci, Gillian M.; Michejda, Christopher J.] NCI, Mol Aspects Drug Design Sect, Struct Biophys Lab, Ctr Canc Res, Frederick, MD 21702 USA. [Keay, Susan K.; Koch, Kristopher R.; Zhang, Chen-Ou, Grkovic, David, Guo, Li] Univ Maryland, Sch Med, Dept Med, Div Infect Dis, Baltimore, MD 21201 USA. [Keay, Susan K.] Vet Adm Maryland Hlth Care Syst, Res Serv, Baltimore, MD 21201 USA. [Barchi, Joseph J., Jr.] NCI, Med Chem Lab, Ctr Canc Res, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Journal of Medicinal Chemistry
    1. 51
    2. 19
    3. Pages: 5974-5983
  4. Type of Article: Article
  1. Abstract:

    We performed comprehensive structure-activity relationship (SAR) studies on the peptide portion of antiproliferative factor (APF), a sialylated frizzled-8 related glycopeptide that inhibits normal bladder epithelia] and urothelial carcinoma cell proliferation. Glycopeptide derivatives were synthesized by solid-phase methods using standard Fmoc chemistry and purified by RP-HPLC, all intermediate and final products were verified by HPLC-MS and NMR analyses. Antiproliferative activity of each derivative was determined by inhibition of H-3-thymidine incorporation in primary normal human bladder epithelial cells. Structural components of the peptide segment of APF that proved to be important for biological activity included the presence of at least eight of the nine N-terminal amino acids, a negative charge in the C-terminal amino acid, a free amino group at the N-terminus, maintenance of a specific amino acid sequence in the C-terminal tail, and trans conformation for the peptide bonds. These data provide critical guidelines for optimization of structure in design of APF analogues as potential therapeutic agents.

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External Sources

  1. PMID: 18788730

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