Skip NavigationSkip to Content
Return Return to Search Results

Neurocalcin delta modulation of ROS-GC1, a new model of Ca2+ signaling

  1. Author:
    Venkataraman, V.
    Duda, T.
    Ravichandran, S.
    Sharma, R. K.

  2. Author Address

    Duda, Teresa, Sharma, Rameshwar K.] Penn Coll Optometry, Div Biochem & Mol Biol, Unit Regulatory & Mol Biol, Elkins Pk, PA 19027 USA. [Venkataraman, Venkateswar] Univ Med & Dent New Jersey, Sch Osteopath Med, Dept Cell Biol, Stratford, NJ 08084 USA. [Ravichandran, Sarangan] SAIC Frederick, Natl Canc Inst, Adv Biomed Computing Ctr, Frederick, MD 21702 USA.
    1. Year: 2008
  2. Journal: Biochemistry
    1. 47
    2. 25
    3. Pages: 6590-6601
  4. Type of Article: Article
  1. Abstract:

    ROS-GC1 membrane guanylate cyclase is a Ca2+ bimodal signal transduction switch. It is turned "off" by a rise in free Ca2+ from nanomolar to the semicromolar range in the photoreceptor outer segments and the olfactory bulb neurons, by a similar rise in the bipolar and ganglion retinal neurons it is turned "on". These opposite operational modes of the switch are specified by its Ca2+ sensing devices, respectively termed GCAPs and CD-GCAPs. Neurocalcin delta is a CD-GCAP. In the present study, the neurocalcin delta-modulated site, V-837-L-858, in ROS-GC1 has been mapped. The location and properties of this site are unique. It resides within the core domain of the catalytic module and does not require the alpha-helical dimerization domain structural element (amino acids 767-811) for activating the catalytic module. Contrary to the current beliefs, the catalytic module is intrinsically active, it is directly regulated by the neurocalcin delta-modulated Ca2+ signal and is dimeric in nature. A fold recognition based model of the catalytic domain of ROS-GC1 was built, and neurocalcin delta docking simulations were carried out to define the three-dimensional features of the interacting domains of the two molecules. These findings define a new transduction model for the Ca2+ signaling of ROS-GC1.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel