Skip NavigationSkip to Content
Return Return to Search Results

Inhibition of human immunodeficiency virus type 1 assembly and release by the cholesterol-binding compound amphotericin B methyl ester: Evidence for Vpu dependence

  1. Author:
    Waheed, A. A.
    Ablan, S. D.
    Soheilian, F.
    Nagashima, K.
    Ono, A.
    Schaffner, C. P.
    Freed, E. O.

  2. Author Address

    Waheed, Abdul A.; Ono, Akira, Freed, Eric O.] NCI, HIV Drug Resistance Program, Virus Cell Interact Sect, Frederick, MD 21702 USA. [Soheilian, Ferri, Nagashima, Kunio] NCI, SAIC Frederick, Res Technol Program, Image Anal Lab, Frederick, MD 21702 USA. [Schaffner, Carl P.] Rutgers State Univ, Waksman Inst, Dept Microbiol & Biochem, New Brunswick, NJ 08903 USA.
    1. Year: 2008
  2. Journal: Journal of Virology
    1. 82
    2. 19
    3. Pages: 9776-9781
  4. Type of Article: Article
  1. Abstract:

    We investigated the mechanism by which the cholesterol- binding compound amphotericin B methyl ester (AME) inhibits human immunodeficiency virus type 1 (HIV-1) particle production. We observed no significant effect of AME on Gag binding to the plasma membrane, Gag association with lipid rafts, or Gag multimerization, indicating that the mechanism of inhibition by AME is distinct from that by cholesterol depletion. Electron microscopy analysis indicated that AME significantly disrupts virion morphology. Interestingly, we found that AME does not inhibit the release of Vpu-defective HIV-1 or Vpu(-) retroviruses such as murine leukemia virus and simian immunodeficiency virus. We demonstrated that the ability of Vpu to counter the activity of CD317/BST-2/tetherin is markedly reduced by AME. These results indicate that AME interferes with the anti-CD317/BST-2/tetherin function of Vpu.

    See More

  1. Keywords:

External Sources

  1. PMID: 18653459

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel