Skip NavigationSkip to Content
Return Return to Search Results

Human papillomavirus false positive cytological diagnosis in low grade squamous intraepithelial lesion

  1. Author:
    Nunez-Troconis, J.
    Delgado, M.
    Gonzalez, J.
    Velasquez, J.
    Mindiola, R.
    Whitby, D.
    Conde, B.
    Munroe, D. J.

  2. Author Address

    Nunez-Troconis, Jose] Univ Zulia, Hosp Manuel Noriega Trigo, Dept Obstet & Ginecol, Fac Med, Maracaibo 4011, Venezuela. [Delgado, Mariela, Gonzalez, Julia] Policlin Maracaibo, Lab Patol, Maracaibo, Venezuela. [Velasquez, Jesvy, Mindiola, Raimy] Univ Zulia, Fac Med, Lab Reg Referencia Virol, Maracaibo 4011, Venezuela. [Whitby, Denise, Conde, Betty] NCI, Viral Oncol Sect, Core Lab, SAIC Frederick Inc, Frederick, MD 21701 USA. [Munroe, David J.] NCI, Lab Mol Technol, SAIC Frederick Inc, Frederick, MD 21701 USA.
    1. Year: 2009
  2. Journal: Investigacion Clinica
    1. 50
    2. 4
    3. Pages: 447-454
  4. Type of Article: Article
  5. ISSN: 0535-5133
  1. Abstract:

    The purpose of this study was to investigate the number of Human Papilloma-virus false positive cytological diagnosis in low grade squamous intraepithelial lesions (LSIL). Three hundred and two women who assisted to an Out-Patient Gynecologic Clinic in Maracaibo, Venezuela, were recruited for this study. Each patient had the Pap smear and a cervical swab for Hybrid Capture 2 (HC2). Three cytotechnologists reviewed the Pap smears and two pathologists rescreened all of them. The cytotechnologists reported 161 (53.3%) Pap smears negatives for intraepithelial lesion (IL) or malignancy, and 141 cases (46.7%) with epithelial abnormalities. They reported 46% of 302 patients with HPV infection in Pap smear slides. The pathologists found that 241 (79.8%) Pap smears were negatives for IL or malignancy and 61 (20.2%), with abnormal Pap smears. They found 14.6% HPV infection in all Pap smears (p<0.0001, 46% vs 14.6%). The HC2 study showed that 47 samples (15.6%) were positive for HPV. The study found that 114 Pap smears (False Positive: 85%) of 134 reported by the cytotechnologists and 24 (False Positive: 43%) of 56 cytologies reported by the pathologists as LSIL, were negative for HPV infection determined by HC2 (p<0.00003). The present study suggests that the cytotechnologists overdiagnosed cellular changes associated with HPV infection in the Pap smear, increasing the FP cytological diagnosis of LSIL.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel