Skip NavigationSkip to Content
Return Return to Search Results

Lsh Mediated RNA Polymerase II Stalling at HoxC6 and HoxC8 Involves DNA Methylation

  1. Author:
    Tao, Y. G.
    Xi, S. C.
    Briones, V.
    Muegge, K.

  2. Author Address

    [Tao, Yongguang; Xi, Sichuan; Briones, Victorino; Muegge, Kathrin] NCI, Lab Canc Prevent, SAIC Frederick, Frederick, MD 21701 USA.;Tao, YG, NCI, Lab Canc Prevent, SAIC Frederick, Frederick, MD 21701 USA.;muegge@ncifcrf.gov
    1. Year: 2010
    2. Date: Feb
    3. Epub Date: 2/18/2010
  2. Journal: Plos One
    1. 5
    2. 2
    3. Pages: e9163
  4. Type of Article: Article
  5. Article Number: e9163
  6. ISSN: 1932-6203
  1. Abstract:

    DNA cytosine methylation is an important epigenetic mechanism that is involved in transcriptional silencing of developmental genes. Several molecular pathways have been described that interfere with Pol II initiation, but at individual genes the molecular mechanism of repression remains uncertain. Here, we study the molecular mechanism of transcriptional regulation at Hox genes in dependence of the epigenetic regulator Lsh that controls CpG methylation at selected Hox genes. Wild type cells show a nucleosomal deprived region around the transcriptional start site at methylated Hox genes and mediate gene silencing via Pol II stalling. Hypomethylation in Lsh-/- cells is associated with efficient transcriptional elongation and splicing, in part mediated by the chromodomain protein Chd1. Dynamic modulation of DNA methylation in Lsh-/- and wild type cells demonstrates that catalytically active DNA methyltransferase activity is required for Pol II stalling. Taken together, the data suggests that DNA methylation can be compatible with Pol II binding at selected genes and Pol II stalling can act as alternate mechanism to explain transcriptional silencing associated with DNA methylation.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1371/journal.pone.0009163
  3. PMID: 20161795
  4. PMCID: PMC2820093
  5. View record in Web of Science®
  6. WOS: 000274442800009

Library Notes

  1. Fiscal Year: FY2009-2010
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel