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The impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25-hydroxyvitamin D concentrations

  1. Author:
    Yu, C. L.
    Falk, R. T.
    Kimlin, M. G.
    Rajaraman, P.
    Sigurdson, A. J.
    Horst, R. L.
    Cosentino, L. M.
    Linet, M. S.
    Freedman, D. M.

  2. Author Address

    [Yu, Chu-Ling; Falk, Roni T.; Rajaraman, Preetha; Sigurdson, Alice J.; Linet, Martha S.; Freedman, D. Michal] NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, Rockville, MD 20852 USA. [Kimlin, Michael G.] Queensland Univ Technol, Inst Hlth & Biomed Innovat, Australian Sun & Hlth Res Lab, Brisbane, Qld 4001, Australia. [Horst, Ronald L.] Heartland Assays Inc, Ames, IA USA. [Cosentino, Louis M.] NCI, Lab Biospecimen & Biorepository Res, SAIC Frederick Inc, Frederick, MD 21701 USA.;Yu, CL, NCI, Div Canc Epidemiol & Genet, NIH, Dept Hlth & Human Serv, 6120 Execut Blvd,Mailstop 7238, Rockville, MD 20852 USA.;yuchu@mail.nih.gov
    1. Year: 2010
    2. Date: Apr
  2. Journal: Cancer Causes & Control
    1. 21
    2. 4
    3. Pages: 643-648
  4. Type of Article: Article
  5. ISSN: 0957-5243
  1. Abstract:

    Studies have examined the associations between cancers and circulating 25-hydroxyvitamin D [25(OH)D], but little is known about the impact of different laboratory practices on 25(OH)D concentrations. We examined the potential impact of delayed blood centrifuging, choice of collection tube, and type of assay on 25(OH)D concentrations. Blood samples from 20 healthy volunteers underwent alternative laboratory procedures: four centrifuging times (2, 24, 72, and 96 h after blood draw); three types of collection tubes (red top serum tube, two different plasma anticoagulant tubes containing heparin or EDTA); and two types of assays (DiaSorin radioimmunoassay [RIA] and chemiluminescence immunoassay [CLIA/LIAISON(A (R))]). Log-transformed 25(OH)D concentrations were analyzed using the generalized estimating equations (GEE) linear regression models. We found no difference in 25(OH)D concentrations by centrifuging times or type of assay. There was some indication of a difference in 25(OH)D concentrations by tube type in CLIA/LIAISON(A (R))-assayed samples, with concentrations in heparinized plasma (geometric mean, 16.1 ng ml(-1)) higher than those in serum (geometric mean, 15.3 ng ml(-1)) (p = 0.01), but the difference was significant only after substantial centrifuging delays (96 h). Our study suggests no necessity for requiring immediate processing of blood samples after collection or for the choice of a tube type or assay.

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External Sources

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  2. DOI: 10.1007/s10552-009-9485-x
  3. View record in Web of Science®
  4. WOS: 000275631900014

Library Notes

  1. Fiscal Year: FY2009-2010
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