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Sequences required for transcription termination at the intrinsic lambda tI terminator

  1. Author:
    Martinez-Trujillo, M.
    Sanchez-Trujillo, A.
    Ceja, V.
    Avila-Moreno, F.
    Bermudez-Cruz, R. M.
    Court, D.
    Montanez, C.

  2. Author Address

    [Martinez-Trujillo, Miguel; Sanchez-Trujillo, Alejandra; Ceja, Victor; Avila-Moreno, Federico; Maria Bermudez-Cruz, Rosa; Montanez, Cecilia] Ctr Invest & Estudios Avanzados IPN, Dept Genet & Biol Mol, Mexico City 07360, DF, Mexico. [Court, Donald] NCI, Frederick, MD 21702 USA.;Montanez, C, Ctr Invest & Estudios Avanzados IPN, Dept Genet & Biol Mol, Apartado Postal 14-740, Mexico City 07360, DF, Mexico.;cecim@cinvestav.mx
    1. Year: 2010
    2. Date: Feb
  2. Journal: Canadian Journal of Microbiology
    1. 56
    2. 2
    3. Pages: 168-177
  4. Type of Article: Article
  5. ISSN: 0008-4166
  1. Abstract:

    The lambda tI terminator is located approximately 280 bp beyond the lambda int gene, and it has a typical structure of an intrinsic terminator. To identify sequences required for lambda tI transcription termination a set of deletion mutants were generated, either from the 5' or the 3' end onto the lambda tI region. The termination efficiency was determined by measuring galactokinase (galK) levels by Northern blot assays and by in vitro transcription termination. The importance of the uridines and the stability of the stem structure in the termination were demonstrated. The nontranscribed DNA beyond the 3' end also affects termination. Additionally, sequences upstream have a small effect on transcription termination. The in vivo RNA termination sites at lambda tI were determined by S1 mapping and were located at 8 different positions. Processing of transcripts from the 3' end confirmed the importance of the hairpin stem in protection against exonuclease.

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  2. DOI: 10.1139/w09-123
  3. View record in Web of Science®
  4. WOS: 000275986900008

Library Notes

  1. Fiscal Year: FY2009-2010
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