Potential Mentors for 2020 - 2021

C

Elena Cornejo Castro

Research Goals/Purpose:

The Viral Oncology Section (VOS) has developed a genome capture approach for the generation of Kaposi's sarcoma herpesvirus (KSHV) whole genome sequences using next generation sequencing. This virus is the causative agent of multiple malignancies observed throughout the world. Previously, very limited genetic characterization of KSHV was possible and strains were categorized based on few genes.

Training Plan:

The generation, characterization and downstream analysis of these data requires the use and adaptation of open-source bioinformatic tools. The student will assist in the comparative analysis of herpesvirus genomes, including the identification of sequence variations and recombination analysis. Further the student will validate and if necessary adapt a newly developed computational pipeline for the assembly of viral genomes.

F

John Fenimore

Research Goals/Purpose:

Our lab specializes in cancer and inflammation biology, with a focus in the cytokine, interferon gamma. It will also be a vital duty of our students to assist in helping support our mouse colony via regular genotyping and other lab duties. My work focuses on mitochondrial defects in our mouse model and how it related to human diseases with examinations of fatigue, metabolic and functional changes. Our students will work with model cell lines to examine underlying factors and questions in our inflammatory model.

Training Plan:

Along with basic laboratory skills, we hope to promote critical thinking and other tasks that will answer questions regarding this model of disease. We wish to promote a feeling of inclusion in our lab. Instruction on genotyping, cell culture, staining and working with your mentor on experimental design will be a large part of your experience.

K

Suhas Kharat

Research Goals/Purpose:

Student will learn basic molecular biology techniques such as plasmid isolation, primer designing, PCR reaction setup, restriction digestion, DNA ligation, bacterial transformation. Apart from molecular biology students will also work on some bioinformatic assignments such as analyzing DNA and protein sequences in BLAST and ClustalW tools. As part of specialized training students will learn fluorescence microscopy.

Training Plan:

Similar to Research goals.