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Efficient induction of CD25(-) iTreg by co-immunization requires strongly antigenic epitopes for T cells

  1. Author:
    Geng, S. A.
    Yu, Y.
    Kang, Y. M.
    Pavlakis, G.
    Jin, H. L.
    Li, J. Y.
    Hu, Y. X.
    Hu, W. B.
    Wang, S. A.
    Wang, B.

  2. Author Address

    [Geng, SA; Yu, Y; Kang, YM; Jin, HL; Li, JY; Hu, WB; Wang, SA; Wang, B] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China [Pavlakis, G] NCI, Ctr Canc Res, Frederick, MD 21702 USA [Hu, YX] China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China [Jin, HL] Univ Illinois, Coll Med, Dept Microbiol & Immunol, Chicago, IL 60612 USA;Wang, SAWang, B (reprint author), China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China;bwang03@gmail.com
    1. Year: 2011
    2. Date: May
  2. Journal: Bmc Immunology
    1. 12
    2. Pages: 9
  4. Type of Article: Article
  5. Article Number: 27
  6. ISSN: 1471-2172
  1. Abstract:

    Background: We previously showed that co-immunization with a protein antigen and a DNA vaccine coding for the same antigen induces CD40(low) IL-10(high) tolerogenic DCs, which in turn stimulates the expansion of antigen-specific CD4(+) CD25-Foxp3(+) regulatory T cells (CD25(-) iTreg). However, it was unclear how to choose the antigen sequence to maximize tolerogenic antigen presentation and, consequently, CD25(-) iTreg induction. Results: In the present study, we demonstrated the requirement of highly antigenic epitopes for CD25(-) iTreg induction. Firstly, we showed that the induction of CD25(-) iTreg by tolerogenic DC can be blocked by anti-MHC-II antibody. Next, both the number and the suppressive activity of CD25(-) iTreg correlated positively with the overt antigenicity of an epitope to activate T cells. Finally, in a mouse model of dermatitis, highly antigenic epitopes derived from a flea allergen not only induced more CD25(-) iTreg, but also more effectively prevented allergenic reaction to the allergen than did weakly antigenic epitopes. Conclusions: Our data thus indicate that efficient induction of CD25(-) iTreg requires highly antigenic peptide epitopes. This finding suggests that highly antigenic epitopes should be used for efficient induction of CD25(-) iTreg for clinical applications such as flea allergic dermatitis.

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External Sources

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  2. DOI: 10.1186/1471-2172-12-27
  3. View record in Web of Science®
  4. WOS: 000291267200001

Library Notes

  1. Fiscal Year: FY2010-2011
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