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Majority of CD4(+) T cells from peripheral blood of HIV-1-infected individuals contain only one HIV DNA molecule

  1. Author:
    Josefsson, L.
    King, M. S.
    Makitalo, B.
    Brannstrom, J.
    Shao, W.
    Maldarelli, F.
    Kearney, M. F.
    Hu, W. S.
    Chen, J. B.
    Gaines, H.
    Mellors, J. W.
    Albert, J.
    Coffin, J. M.
    Palmer, S. E.

  2. Author Address

    [Josefsson, L; Makitalo, B; Brannstrom, J; Gaines, H; Palmer, SE] Swedish Inst Infect Dis Control, Dept Diagnost & Vaccinol, S-17182 Solna, Sweden [Josefsson, L; Albert, J; Palmer, SE] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, S-17182 Solna, Sweden [Josefsson, L; Maldarelli, F; Kearney, MF; Hu, WS; Chen, JB; Coffin, JM; Palmer, SE] NCI, HIV Drug Resistance Program, Frederick, MD 21702 USA [King, MS] Abbott Labs, Global Pharmaceut Res & Dev, Abbott Pk, IL 60069 USA [Shao, W] SAIC Frederick Inc, Adv Biomed Comp Ctr, Frederick, MD 21702 USA [Mellors, JW] Univ Pittsburgh, Dept Med, Div Infect Dis, Pittsburgh, PA 15261 USA;Josefsson, L (reprint author), Swedish Inst Infect Dis Control, Dept Diagnost & Vaccinol, S-17182 Solna, Sweden;Lina.Josefsson@smi.se john.coffin@tufts.edu
    1. Year: 2011
    2. Date: Jul
  2. Journal: Proceedings of the National Academy of Sciences of the United States of America
    1. 108
    2. 27
    3. Pages: 11199-11204
  4. Type of Article: Article
  5. ISSN: 0027-8424
  1. Abstract:

    Neither the number of HIV-1 proviruses within individual infected cells in HIV-1-infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a technique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4(+) T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, implying a limited potential for recombination in virus produced by these cells. The genetic similarity between HIV populations in CD4(+) T cells and plasma implies ongoing exchange between these compartments both early and late after infection.

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External Sources

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  2. DOI: 10.1073/pnas.1107729108
  3. View record in Web of Science®
  4. WOS: 000292376700056

Library Notes

  1. Fiscal Year: FY2010-2011
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