Skip NavigationSkip to Content
Return Return to Search Results

Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity

  1. Author:
    Kawai, T.
    Nomura, F.
    Hoshino, K.
    Copeland, N. G.
    Gilbert, D. J.
    Jenkins, N. A.
    Akira, S.

  2. Author Address

    Akira S Hyogo Coll Med, Dept Biochem 1-1 Mukogawa Cho Nishinomiya Hyogo 6638501 Japan Hyogo Coll Med, Dept Biochem Nishinomiya Hyogo 6638501 Japan NCI, Mammalian Genet Lab, ABL Basic Res Program, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA Japan Sci & Technol Corp, CREST Tokyo Japan
    1. Year: 1999
  2. Journal: Oncogene
    1. 18
    2. 23
    3. Pages: 3471-3480
  4. Type of Article: Article
  1. Abstract:

    We have identified and characterized a new calcium/calmodulin (Ca2+/CaM) dependent protein kinase termed death-associated protein kinase 2 (DAPK2) that contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis, DAPK2 mRNA is expressed abundantly in heart, lung and skeletal muscle. The mapping results indicated that DAPK2 is located in the central region of mouse chromosome 9, bl vitro kinase assay revealed that DAPK2 is autophosphorylated and phosphorylates myosin light chain (MLC) as an exogenous substrate. DAPK2 binds directly to CaM and is activated in a Ca2+/CaM-dependent manner. A constitutively active DAPK2 mutant is generated by removal of the CaM-binding domain (Delta CaM). Treatment of agonists that elevate intracellular Ca2+-concentration led to the activation of DAPK2 and transfection studies revealed that DAPK2 is localized in the cytoplasm. Overexpression of DAPK2, but not the kinase negative mutant, significantly induced the morphological changes characteristic of apoptosis, These results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling. [References: 45]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel