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Fine mapping of the friend retrovirus resistance gene, Rfv3, on mouse chromosome 15

  1. Author:
    Super, K. J.
    Hasenkrug, K. J.
    Simmons, S.
    Brooks, D. M.
    Konzek, R.
    Sarge, K. D.
    Morimoto, R. I.
    Jenkins, N. A.
    Gilbert, D. J.
    Copeland, N. G.
    Frankel, W.
    Chesebro, B.

  2. Author Address

    Chesebro B NIAID, Rocky Mt Labs, Persistent Viral Dis Lab, NIH 903 S 4th St Hamilton, MT 59840 USA NIAID, Rocky Mt Labs, Persistent Viral Dis Lab, NIH Hamilton, MT 59840 USA Univ Kentucky, Dept Biochem Lexington, KY 40536 USA Northwestern Univ, Dept Biochem Mol Biol & Cell Biol Evanston, IL 60208 USA NCI, Mammalian Genet Lab, ABL Basic Res Program, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA Jackson Lab Bar Harbor, ME 04609 USA
    1. Year: 1999
  2. Journal: Journal of Virology
    1. 73
    2. 9
    3. Pages: 7848-7852
  4. Type of Article: Article
  1. Abstract:

    Rfv3 is a host resistance gene that operates through an unknown mechanism to control the development of the virus-neutralizing antibody response required for recovery from infection with Friend retrovirus. The Rfv3 gene was previously mapped to an approximately 20-centimorgan (cM) region of chromosome 15. More refined mapping was not possible, due to a lack of microsatellite markers and leakiness in the Rfv3 phenotype, which prevented definitive phenotyping of individual recombinant mice. In the present study, we overcame these difficulties by taking advantage of seven new microsatellite markers in the Rfv3 region and by using progeny tests to accurately determine the Rfv3 phenotype of recombinant mice. Detailed linkage analysis of relevant crossovers narrowed the location of Rfv3 to a 0.83-cM region. Mapping of closely linked genes in an interspecific backcross panel allowed us to exclude two previous candidate genes, Ly6 and Wnt7b. These studies also showed for the first time that the Hsf1 gene maps to the Rfv3-linked cluster of genes including Il2rb, Il3rb, and Pdgfb. This localization of Rfv3 to a region of less than 1 cM now makes it feasible to attempt the cloning of Rfv3 by physical methods. [References: 22]

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