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MGA2 or SPT23 is required for transcription of the Delta 9 fatty acid desaturase gene, OLE1, and nuclear membrane integrity in Saccharomyces cerevisiae

  1. Author:
    Zhang, S. R.
    Skalsky, Y.
    Garfinkel, D. J.

  2. Author Address

    Garfinkel DJ NCI, Movable Genet Elements Sect, Gene Regulat & Chromosome Biol Lab,Adv BioSci Lab, Basic Res Program,Frederick Canc Res & Dev Ctr POB B Ft Detrick, MD 21702 USA NCI, Movable Genet Elements Sect, Gene Regulat & Chromosome Biol Lab,Adv BioSci Lab, Basic Res Program,Frederick Canc Res & Dev Ctr Ft Detrick, MD 21702 USA
    1. Year: 1999
  2. Journal: Genetics
    1. 151
    2. 2
    3. Pages: 473-483
  4. Type of Article: Article
  1. Abstract:

    MGA2 and SPT23 are functionally and genetically redundant homologs in Saccharomyces cerevisiae. Both genes are implicated in the transcription of a subset of genes, including Ty retrotransposons and Ty-induced mutations. Neither gene is essential for growth, but mga2 spt23 double mutants are inviable. We have isolated a gene-specific activator, SW15, and the Delta 9 fatty acid desaturase of yeast, OLE1, as multicopy suppressors of an mga2 Delta spt23 temperature-sensitive mutation (spt23-ts). The le iel of unsaturated fatty acids decreases 35-40% when the mga2 Delta spt23-ts mutant is incubated at 37 degrees. Electron microscopy of these cells reveals a separation of inner and outer nuclear membranes that is sometimes accompanied by vesiclelike projections in the intermembrane space. The products of Ole1p catalysis, oleic acid and palmitoleic acid, suppress mga2 Delta spt23-ts and mga2 Delta spt23 Delta lethality and restore normal nuclear membrane morphology. Furthermore, the level of the OLE1 transcript decreases more than 15-fold in the absence of wild-type Mga2p and Spt23p. Our results suggest that Mga2p/Spt23p control cell viability by stimulating OLE1 transcription. [References: 49]

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