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Escherichia coli nusG mutations that block transcription termination by coliphage HK022 Nun protein

  1. Author:
    Burova, E.
    Hung, S. C.
    Chen, J.
    Court, D. L.
    Zhou, J. G.
    Mogilnitskiy, G.
    Gottesman, M. E.

  2. Author Address

    Gottesman ME Columbia Univ Coll Phys & Surg, Inst Canc Res 701 W 168th St New York, NY 10032 USA Columbia Univ Coll Phys & Surg, Inst Canc Res New York, NY 10032 USA Columbia Univ Coll Phys & Surg, Dept Biochem & Mol Biophys New York, NY 10032 USA NCI, Lab Gene Regulat & Chromosome Biol, ABL Basic Res Program, Frederick Canc Res & Dev Ctr Frederick, MD 21701 USA
    1. Year: 1999
  2. Journal: Molecular Microbiology
    1. 31
    2. 6
    3. Pages: 1783-1793
  4. Type of Article: Article
  1. Abstract:

    The Escherichia coli nusG gene product is required for transcription termination by phage HK022 Nun protein at the lambda nutR site in vivo. We show that it is also essential for Nun termination at lambda nutL. Three recessive missense nusG mutations have been isolated that inhibit termination by Nun at lambda nutR. The mutations are ineffective in a lambda pL nutL fusion, even when lambda nutR replaces lambda nutL. The mutant strains support lambda growth, indicating that lambda N antitermination activity is not impaired. Transcription arrest by Nun in vitro is stimulated by NusG protein at both lambda nutR and lambda nutL. Mutant NusG protein fails to enhance transcriptional arrest by Nun at either site. The mutant protein, like the wild-type protein, suppresses transcriptional pausing by RNA polymerase and stimulates Rho-dependent termination. These results imply that the role of NusG in Nun termination may be distinct from its roles in other transcription reactions. [References: 39]

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