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Immunization against SIVmne in macaques using multigenic DNA vaccines

  1. Author:
    Mossman, S. P.
    Pierce, C. C.
    Robertson, M. N.
    Watson, A. J.
    Montefiori, D. C.
    Rabin, M.
    Kuller, L.
    Thompson, J.
    Lynch, J. B.
    Morton, W. R.
    Benveniste, R. E.
    Munn, R.
    Hu, S. L.
    Greenberg, P.
    Haigwood, N. L.

  2. Author Address

    Haigwood NL Seattle Biomed Res Inst 4 Nickerson St Seattle, WA 98109 USA Seattle Biomed Res Inst Seattle, WA 98109 USA Univ Washington, Dept Med Seattle, WA USA Duke Univ, Med Ctr Durham, NC USA Univ Washington, Washington Reg Primate Res Ctr Seattle, WA 98195 USA NCI, Viral Carcinogenesis Lab Frederick, MD USA Univ Calif Davis, Sch Med Davis, CA 95616 USA
    1. Year: 1999
  2. Journal: Journal of Medical Primatology
    1. 28
    2. 4-5
    3. Pages: 206-213
  4. Type of Article: Article
  1. Abstract:

    All structural and regulatory genes of SIVmne were cloned into mammalian expression vectors to optimize expression in vitro and immunogenicity in mice. Macaca fascicularis were immunized four times with plasmid DNA (n = 4), or two DNA priming inoculations followed by two boosts of recombinant gp160 plus Gag-Pol particles (n = 4). Following intrarectal challenge with SIVmne, all macaques be came infected. Three monkeys immunized with DNA alone maintained low plasma virus loads by 1 year post-challenge; the fourth exhibited high virus loads and significant CD4(+) cell decline. Two of the DNA plus boost and three control macaques had high virus loads and associated CD4(+) cell decline. Both vaccine protocols elicited antibodies and comparable helper T-cell proliferative responses to gp160. Cytokine mRNA levels in activated peripheral blood mononuclear cells (PBMC) takes at time of challenge suggested a dominant T helper (Th) 1 state in three DNA-immunized and one protein-boosted macaque, which correlated with low virus loads and high CD4(+) cell counts post-challenge. [References: 23]

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