Skip NavigationSkip to Content
Return Return to Search Results

Analysis of NotI linking clones isolated from human chromosome 3 specific libraries

  1. Author:
    Kashuba, V. I.
    Gizatullin, R. Z.
    Protopopov, A. I.
    Li, J.
    Vorobieva, N. V.
    Fedorova, L.
    Zabarovska, V. I.
    Muravenko, O. V.
    Kost-Alimova, M.
    Domninsky, D. A.
    Kiss, C.
    Allikmets, R.
    Zakharyev, V. M.
    Braga, E. A.
    Sumegi, J.
    Lerman, M.
    Wahlestedt, C.
    Zelenin, A. V.
    Sheer, D.
    Winberg, G.
    Grafodatsky, A.
    Kisselev, L. L.
    Klein, G.
    Zabarovsky, E. R.

  2. Author Address

    Kashuba VI Karolinska Inst, Ctr Microbiol & Tumor Biol S-17177 Stockholm Sweden Karolinska Inst, Ctr Microbiol & Tumor Biol S-17177 Stockholm Sweden Karolinska Inst, Ctr Genom Res S-17177 Stockholm Sweden Ukrainian Acad Sci, Inst Mol Biol & Genet UA-252627 Kiev Ukraine Russian Acad Sci, Siberian Branch, Inst Cytol & Genet Novosibirsk 630090 Russia Russian Acad Sci, VA Engelhardt Mol Biol Inst Moscow 117984 Russia NCI, Intramural Res Support Program, SAIC Frederick, FCRDC Ft Detrick, MD 21702 USA Russian State Genet Ctr Moscow 113545 Russia Univ Nebraska, Med Ctr, Dept Pathol & Microbiol Omaha, NE 68198 USA NCI, FCRDC Ft Detrick, MD 21702 USA Imperial Canc Res Fund London WC2A 3PX England
    1. Year: 1999
  2. Journal: Gene
    1. 239
    2. 2
    3. Pages: 259-271
  4. Type of Article: Article
  1. Abstract:

    We have partially sequenced more than 1000 NotI linking clones isolated from human chromosome S-specific libraries. Of these clones, 152 were unique chromosome 3-specific clones. The clones were precisely mapped using a combination of fluorescence in situ hybridization (FISH) and hybridization to somatic cell or radiation hybrids. Two- and three-color FISH was used to order the clones that mapped to the same chromosomal region, and in some cases, chromosome jumping was used to resolve ambiguous mapping. When this NotI restriction map was compared with the yeast artificial chromosome (YAC) based chromosome 3 map, significant differences in several chromosome 3 regions were observed. A search of the EMBL, nucleotide database with these sequences revealed homologies (90-100%) to more than 100 different genes or expressed sequence tags (ESTs). Many of these homologies were used to map new genes to chromosome 3. These results suggest that sequencing NotI linking clones, and sequencing CpG islands in general, may complement the EST project and aid in the discovery of all human genes by sequencing random cDNAs. This method may also yield information that cannot be obtained by the EST project alone; namely, the identification of the 5' ends of genes, including potential promoter/enhancer regions and other regulatory sequences (C) 1999 Elsevier Science B.V. All rights reserved. [References: 36]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel