Skip NavigationSkip to Content
Return Return to Search Results

Analysis of receptor usage by ecotropic murine retroviruses, using green fluorescent protein-tagged cationic amino acid transporters

  1. Author:
    Masuda, M.
    Kakushima, N.
    Wilt, S. G.
    Ruscetti, S. K.
    Hoffman, P. M.
    Iwamoto, A.

  2. Author Address

    Masuda M Univ Tokyo, Dept Microbiol, Grad Sch Med, Bunkyo Ku 7-3-1 Hongo Tokyo 1130033 Japan Univ Tokyo, Dept Microbiol, Grad Sch Med, Bunkyo Ku Tokyo 1130033 Japan Univ Tokyo, Inst Med Sci, Dept Infect Dis Tokyo 1088639 Japan Dept Vet Affairs Med Ctr, Res Serv Baltimore, MD 21201 USA NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci Frederick, MD 21702 USA
    1. Year: 1999
  2. Journal: Journal of Virology
    1. 73
    2. 10
    3. Pages: 8623-8629
  4. Type of Article: Article
  1. Abstract:

    Entry of ecotropic murine leukemia virus (MuLV) into host cells is initiated by interaction between the receptor-binding domain of the viral SU protein and the third extracellular domain (TED) of the receptor, cationic amino acid transporter 1 (CAT1). To study the molecular basis for the retrovirus-receptor interaction, mouse CAT1 (mCAT1) was expressed in human 293 cells as a fusion protein with jellyfish green fluorescent protein (GFP). Easily detected by fluorescence microscopy and immunoblot analysis with anti-GFP antibodies, the mCAT1-GFP fusion protein was expressed in an N-glycosylated form on the cell surface and in the Golgi apparatus, retaining the ecotropic receptor function. The system was applied to compare Friend MuLV (F-MuLV) and its neuropathogenic variant, PVC-211 MuLV, which exhibits a unique cellular tropism and host range, for the ability to use various CAT family members as a receptor. The results indicated that F-MuLV and PVC-211 MuLV could infect the cells expressing wild-type mCAT1 at comparable efficiencies and that rat CAT3, but not mCAT2, conferred a low but detectable level of susceptibility to F-MuLV and PVC-211 MuLV. The data also suggested that CAT proteins might be expressed in an oligomeric form. Further application of the system developed in this study may provide useful insights into the entry mechanism of ecotropic MuLV. [References: 40]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel