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Optimization of Immunostaining for Prospective Image Analysis

  1. Author:
    Lawrence, Scott
    Golubeva, Yelena G

  2. Author Address

    Pharmacodynamics Assay Section (PADIS), Laboratory of Human Toxicology and Pharmacology, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA. Scott.Lawrence@fnlcr.nih.gov., MedImmune, Gaithersburg, MD, USA.,
    1. Year: 2017
  2. Journal: Methods in MolecularBiology
    1. 1606
    2. Pages: 235-263
  4. Type of Article: Article
  5. Article Number: 235-263
  1. Abstract:

    Biomarker discovery is a crucial part of the fast developing field of personalized medicine. Antibody-based techniques including immunostaining of tissue samples are widely used for biomarker evaluation in preclinical and clinical studies. When used in conjunction with robust image analysis methods, it provides a powerful means to assess biomarker modulation, toxicity, and patient response to targeted agents. Here, we describe the optimization of immunofluorescent (IF) staining protocols and a sample IF multiplex protocol suitable for colocalization image analysis.

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  2. DOI: 10.1007/978-1-4939-6990-6_16
  3. PMID: 28502005

Library Notes

  1. Fiscal Year: FY2016-2017
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