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PEG Quantitation Using Reversed-Phase High-Performance Liquid Chromatography and Charged Aerosol Detection

  1. Author:
    Smith, Mackensie C
    Clogston, Jeffrey

  2. Author Address

    Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA., Cancer Research Technology Program, Nanotechnology Characterization Laboratory, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, P.O. Box B, Frederick, MD, 21702, USA. Jeffrey.Clogston@fnlcr.nih.gov.,
    1. Year: 2018
  2. Journal: Methods in molecular biology (Clifton, N.J.)
    1. 1682
    2. Pages: 49-55
  4. Type of Article: Article
  1. Abstract:

    This chapter describes a method for the quantitation of polyethylene glycol (PEG) in PEGylated colloidal gold nanoparticles using a reversed-phase high-performance liquid chromatography (RP-HPLC) with charged aerosol detection. The method can be used to calculate the total PEG on the nanoparticle, as well as the bound and free unbound PEG fractions after a simple centrifugation step. This is a significant distinction as the bound PEG fraction affects biocompatibility, circulation time, and overall nanoparticle efficacy. PEG quantitation can be achieved through two methods, one involving the dissolution of colloidal gold nanoparticles by potassium cyanide (KCN) and the other by displacement of PEG by dithiothreitol (DTT). The methods outlined herein were applied to 30 160;nm colloidal gold grafted with 20 160;kDa PEG, but they can be easily adapted to any size colloidal gold nanoparticle and PEG chain length.

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  2. DOI: 10.1007/978-1-4939-7352-1_5
  3. PMID: 29039092

Library Notes

  1. Fiscal Year: FY2017-2018
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