Partial Proteome of the Corynetoxin-Producing Gram-Positive Bacterium, Rathayibacter toxicus

Author:

Fennessey, Christine

McMahon, Michael B

Sechler, Aaron

Kaiser, Jaclyn

Garrett, WesleyFixed The Affiliations M

Tancos, Matthew A

Luster, Douglas G

Rogers, Elizabeth E

Schneider, William L
Author Address

USDA, ARS, Foreign Disease Weed Science Research Unit, Fort Detrick, Maryland, USA., AIDS and Cancer Virus Program, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, USA., University of Texas Medical Branch, Dept. of Microbiology & Immunology, Galveston, TX, USA., USDA, ARS, Animal Biosciences and Biotechnology Laboratory, Beltsville, Maryland, USA.,

Abstract:

Rathayibacter toxicus is a Gram-positive bacterium that is the causative agent of annual ryegrass toxicity (ARGT), a disease that causes devastating losses in the Australian livestock industry. R. toxicus exhibits a complex life cycle, using the nematode Anguina funesta as a physical vector to carry it up to the seed head of the host plant. ARGT is caused by a tunicamycin-like corynetoxin that is produced in R. toxicus-infected seed galls. We analyzed protein expression in R. toxicus under stationary growth phase conditions to obtain a more complete understanding of the biology of this organism and identify potential targets for immunoassay development. A total of 323 unique proteins were identified, including those with putative roles in secondary metabolism and pathogenicity. The proteome analysis for this complex phytopathogenic Gram-positive bacterium will facilitate in the characterization of proteins necessary for host colonization and toxin production, and assist in the development of diagnostic assays. Data are available via ProteomeXchange with identifier PXD004238. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.

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