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Total drug quantification in prodrugs using an automated elemental analyzer

  1. Author:
    Hu,Yingwen
    Stevens,David
    Man, Sonny
    Crist,Rachael
    Clogston,Jeffrey [ORCID]

  2. Author Address

    Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA., Nanotechnology Characterization Laboratory, Cancer Research Technology Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA. Jeffrey.Clogston@nih.gov.,
    1. Year: 2019
    2. Date: Dec
    3. Epub Date: 2019 05 22
  2. Journal: Drug delivery and translational research
    1. 9
    2. 6
    3. Pages: 1057-1066
  4. Type of Article: Article
  5. ISSN: 2190-393X
  1. Abstract:

    Polymeric prodrugs have become an increasingly popular strategy for improving the pharmacokinetic properties of active pharmaceutical ingredients (API). Therefore, identifying a robust method for quantification of the API in these prodrug products is a key part of the drug development process. Current drug quantification methods include hydrolysis followed by reversed phase high-performance liquid chromatography (RP-HPLC), size exclusion chromatography (SEC)-based molecular weight determination, and mass spectrometry. These methods tend to be time-consuming and often require challenging method development. Here, we present a comparative study highlighting the automated elemental analyzer as a facile approach to drug quantification in this up-and-coming class of therapeutics. A polymeric prodrug using poly(L-lysine succinylated) (PLS) and the drug lamivudine (LAM) was prepared and analyzed using the elemental analyzer in comparison to the traditional approaches of hydrolysis followed by RP-HPLC and SEC using multi-angle light scattering (MALS) detection. The elemental analysis approach showed excellent agreement with the conventional methods but proved much less laborious, highlighting this as a rapid and sensitive analytical method for the quantitative determination of drug loading in polymeric prodrug products.

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External Sources

  1. View Full Text
  2. DOI: 10.1007/s13346-019-00649-8
  3. PMID: 31119521
  4. View record in Web of Science®
  5. WOS: 000497272800004
  6. PII : 10.1007/s13346-019-00649-8

Library Notes

  1. Fiscal Year: FY2018-2019
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