Skip NavigationSkip to Content
Return Return to Search Results

HPV18 Utilizes Two Alternative Branch Sites for E6*I Splicing to Produce E7 Protein

  1. Author:
    Brant, Ayslan Castro
    Majerciak,Vladimir
    Martins Moreira, Miguel Angelo
    Zheng,Zhi-Ming

  2. Author Address

    NCI, Tumor Virus RNA Biol Sect, Biol Lab, Ctr Canc Res,NIH, Frederick, MD 21702 USA.Rio De Janeiro Fed Univ, Genet Post Graduat Program, Rio De Janeiro, Brazil.INCA, Nacl Canc Inst, Genet Program, BR-20231050 Rio De Janeiro, Brazil.
    1. Year: 2019
    2. Date: Apr
    3. Epub Date: 2019 04 03
  2. Journal: Virologica Sinica
  3. SPRINGER,
    1. 34
    2. 2
    3. Pages: 211-221
  5. Type of Article: Article
  6. ISSN: 1674-0769
  1. Abstract:

    Human papillomavirus 18 (HPV18) E6 and E7 oncogenes are transcribed as a single bicistronic E6E7 pre-mRNA. The E6 ORF region in the bicistronic E6E7 pre-mRNA contains an intron. Splicing of this intron disrupts the E6 ORF integrity and produces a spliced E6*I RNA for efficient E7 translation. Here we report that the E6 intron has two overlapped branch point sequences (BPS) upstream of its 3' splice site, with an identical heptamer AACUAAC, for E6*I splicing. One heptamer has a branch site adenosine (underlined) at nt 384 and the other at nt 388. E6*I splicing efficiency correlates to the expression level of E6 and E7 proteins and depends on the selection of which branch site. In general, E6*I splicing prefers the 3'ss-proximal branch site at nt 388 over the distal branch site at nt 384. Inactivation of the nt 388 branch site was found to activate a cryptic acceptor site at nt 636 for aberrant RNA splicing. Together, these data suggest that HPV18 modulates its production ratio of E6 and E7 proteins by alternative selection of the two mapped branch sites for the E6*I splicing, which could be beneficial in its productive or oncogenic infection according to the host cell environment.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1007/s12250-019-00098-0
  3. PMID: 30945125
  4. View record in Web of Science®
  5. WOS: 000468118700009

Library Notes

  1. Fiscal Year: FY2018-2019
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel