Skip NavigationSkip to Content
Return Return to Search Results

Inhibition of tyrosine kinase activation blocks the down-regulation of CXC chemokine receptor 4 by HIV-1 gp120 in CD4(+) T cells

  1. Author:
    Su, S. B.
    Gong, W. H.
    Grimm, M.
    Utsunomiya, I.
    Sargeant, R.
    Oppenheim, J. J.
    Wang, J. M.

  2. Author Address

    Wang JM NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Immunogenet Mol Lab Bldg 560,Room 31-19 Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Immunogenet Mol Lab Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, MD 21702 USA Millenium Biotechnol Ramona, CA 92065 USA
    1. Year: 1999
  2. Journal: Journal of Immunology
    1. 162
    2. 12
    3. Pages: 7128-7132
  4. Type of Article: Article
  1. Abstract:

    Because the binding of HIV-1 envelope to CD4 initiates a cofigurational change in glycoprotein 120 (gp120), enabling it to interact with fusion coreceptors, we investigated how this process interferes with the expression and function of CXC chemokine receptor 4 (CXCR4) in CD4(+) T lymphocytes, A recombinant gp120 (MN), after preincubation with CD4(+) T lymphocytes, significantly inhibited the binding and chemotaxis of the cells in response to the CXCR4 ligand stromal cell-derived factor-1 alpha (SDF-1 alpha), accompanied by a markedly reduced surface expression of CXCR4, gp120, but not SDF-1 alpha, induced rapid tyrosine phosphorylation of src-like kinase p56(lck) in CD4(+) T cells, whereas both gp120 and SDF-1 alpha caused phosphorylation of the CXCR4, The tyrosine kinase inhibitor herbimycin A abolished the phosphorylation of p56(lck) and CXCR4 induced by gp120 in association with maintenance of normal expression of cell surface CXCR4 and a migratory response to SDF-1 alpha, Thus, a CD4-associated signaling molecule(s) including p56(lck) is activated by gp120 and is required for the down-regulation of CXCR4. [References: 30]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel