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Bioorthogonal oncometabolite ligation

  1. Author:
    Briney, Chloe A
    Najera, Susana
    Meier,Jordan

  2. Author Address

    Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, United States., Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, United States. Electronic address: jordan.meier@nih.gov.,
    1. Year: 2019
    2. Epub Date: 2019 03 14
  2. Journal: Methods in enzymology
    1. 622
    2. Pages: 431-448
  4. Type of Article: Article
  5. ISSN: 0076-6879
  1. Abstract:

    Dysregulated cellular metabolism is an emerging hallmark of cancer. Improved methods to profile aberrant metabolic activity thus have substantial applications as tools for diagnosis and understanding the biology of malignant tumors. Here we describe the utilization of a bioorthogonal ligation to fluorescently detect the TCA cycle oncometabolite fumarate. This method enables the facile measurement of fumarate hydratase activity in cell and tissue samples, and can be used to detect disruptions in metabolism that underlie the genetic cancer syndrome hereditary leiomyomatosis and renal cell cancer (HLRCC). The current method has substantial utility for sensitive fumarate hydratase activity profiling, and also provides a foundation for future applications in diagnostic detection and imaging of cancer metabolism. © 2019 Elsevier Inc. All rights reserved.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/bs.mie.2019.02.037
  3. PMID: 31155064
  4. View record in Web of Science®
  5. WOS: 000500350700020
  6. PII : S0076-6879(19)30056-4

Library Notes

  1. Fiscal Year: FY2018-2019
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