Skip NavigationSkip to Content
Return Return to Search Results

Agreement in Histological Assessment of Mitotic Activity Between Microscopy and Digital Whole Slide Images Informs Conversion for Clinical Diagnosis

  1. Author:
    Wei,Bih
    Halsey, Charles H
    Hoover, Shelley B
    Puri, Munish
    Yang, Howard H
    Gallas, Brandon D [ORCID]
    Lee, Maxwell P
    Chen, Weijie
    Durham, Amy C
    Dwyer, Jennifer E
    Sánchez, Melissa D
    Traslavina, Ryan P
    Frank, Chad
    Bradley, Charles
    McGill, Lawrence D
    Esplin, D Glen
    Schaffer, Paula A
    Cramer, Sarah D
    Lyle, L Tiffany
    Beck, Jessica
    Buza, Elizabeth
    Gong, Qi
    Hewitt, Stephen M
    Simpson, R Mark [ORCID]

  2. Author Address

    Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Frederick National Laboratory for Cancer Research, Leidos Biomedical Research, Inc., Frederick, MD, USA., Division of Imaging, Diagnostics, and Software Reliability, Office of Science and Engineering Laboratories, Center for Devices and Radiological Health, US Food and Drug Administration, Silver Spring, MD, USA., Department of Pathobiology, University of Pennsylvania, Philadelphia, PA, USA., Section of Infections of the Nervous System, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA., Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO, USA., Animal Reference Pathology, Salt Lake City, UT, USA., Cancer and Inflammation Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Women 39;s Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Laboratory of Human Carcinogenesis, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.,
    1. Year: 2019
    2. Date: JUL 11
    3. Epub Date: 2019 07 11
  2. Journal: Academic pathology
    1. 6
  4. Type of Article: Article
  5. Article Number: 2374289519859841
  6. ISSN: 2374-2895
  1. Abstract:

    Validating digital pathology as substitute for conventional microscopy in diagnosis remains a priority to assure effectiveness. Intermodality concordance studies typically focus on achieving the same diagnosis by digital display of whole slide images and conventional microscopy. Assessment of discrete histological features in whole slide images, such as mitotic figures, has not been thoroughly evaluated in diagnostic practice. To further gauge the interchangeability of conventional microscopy with digital display for primary diagnosis, 12 pathologists examined 113 canine naturally occurring mucosal melanomas exhibiting a wide range of mitotic activity. Design reflected diverse diagnostic settings and investigated independent location, interpretation, and enumeration of mitotic figures. Intermodality agreement was assessed employing conventional microscopy (CM40 215;), and whole slide image specimens scanned at 20 215; (WSI20 215;) and at 40 215; (WSI40 215;) objective magnifications. An aggregate 1647 mitotic figure count observations were available from conventional microscopy and whole slide images for comparison. The intraobserver concordance rate of paired observations was 0.785 to 0.801; interobserver rate was 0.784 to 0.794. Correlation coefficients between the 2 digital modes, and as compared to conventional microscopy, were similar and suggest noninferiority among modalities, including whole slide image acquired at lower 20 215; resolution. As mitotic figure counts serve for prognostic grading of several tumor types, including melanoma, 6 of 8 pathologists retrospectively predicted survival prognosis using whole slide images, compared to 9 of 10 by conventional microscopy, a first evaluation of whole slide image for mitotic figure prognostic grading. This study demonstrated agreement of replicate reads obtained across conventional microscopy and whole slide images. Hence, quantifying mitotic figures served as surrogate histological feature with which to further credential the interchangeability of whole slide images for primary diagnosis.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1177/2374289519859841
  3. PMID: 31321298
  4. PMCID: PMC6628521
  5. View record in Web of Science®
  6. WOS: 000475433300001
  7. PII : 10.1177_2374289519859841

Library Notes

  1. Fiscal Year: FY2018-2019
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel