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Characterization of and isolation methods for plant leaf nanovesicles and small extracellular vesicles

  1. Author:
    Liu, Yuan
    Wu, Sherry
    Koo, Yeonjong
    Yang, An
    Dai, Yanwan
    Khant,Htet
    Osman, Samantha
    Chowdhury, Mamur
    Wei, Haichao
    Li, Yang
    Court, Karem
    Hwang, Elaine
    Wen, Yunfei
    Dasari, Santosh Kumar
    Nguyen, Michael
    Tang, E Chia-Cheng
    Chehab, E Wassim
    De Val Alda,Natalia
    Braam, Janet
    Sood, Anil K

  2. Author Address

    Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX; BioSciences, Rice University, Houston, TX. Electronic address: yliu32@mdanderson.org., Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: sherry.wu@uq.edu.au., BioSciences, Rice University, Houston, TX. Electronic address: yeonjong@gmail.com., BioSciences, Rice University, Houston, TX; State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing, People 39;s Republic of China. Electronic address: anyang@ibcas.ac.cn., BioSciences, Rice University, Houston, TX. Electronic address: Yanwan.Dai@rice.edu., Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, Frederick, MD; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Inc., Frederick, MD. Electronic address: htet.khant@nih.gov., BioSciences, Rice University, Houston, TX. Electronic address: sro3@rice.edu., Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: MAChowdhury@mdanderson.org., The Vivian L. Smith Department of Neurosurgery, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX. Electronic address: Haichao.Wei@uth.tmc.edu., Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: YLi52@mdanderson.org., Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: karemcourt@gmail.com., BioSciences, Rice University, Houston, TX. Electronic address: esh3@rice.edu., Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: YWen2@mdanderson.org., Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: SKDasari@mdanderson.org., BioSciences, Rice University, Houston, TX. Electronic address: mhn2@rice.edu., BioSciences, Rice University, Houston, TX. Electronic address: ct44@rice.edu., BioSciences, Rice University, Houston, TX. Electronic address: ewchehab@rice.edu., Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, Frederick, MD; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Inc., Frederick, MD. Electronic address: natalia.devalalda@nih.gov., BioSciences, Rice University, Houston, TX. Electronic address: Braam@rice.edu., Department of Gynecologic Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX. Electronic address: asood@mdanderson.org.,
    1. Year: 2020
    2. Date: OCT
    3. Epub Date: 2020 07 20
  2. Journal: Nanomedicine : nanotechnology, biology, and medicine
    1. 29
    2. Pages: 102271
  4. Type of Article: Article
  5. Article Number: 102271
  6. ISSN: 1549-9634
  1. Abstract:

    Mammalian small extracellular vesicles (sEVs) can deliver diverse molecules to target cells. However, they are difficult to obtain in large quantities and can activate host immune responses. Plant-derived vesicles may help to overcome these challenges.We optimized isolation methods for two types of plant vesicles, nanovesicles from disrupted leaf and sEVs from extracellular apoplastic space of Arabidopsis thaliana. Both preparations yielded intact vesicles of uniform size, and a mean membrane charge of approximately -25mV. We also demonstrated applicability of these preparative methods using Brassicaceae vegetables. Proteomic analysis of a subset of vesicles with a density of 1.1-1.19gmL-1 sheds light on the likely cellular origin and complexity of the vesicles. Both leaf nanovesicles and sEVs were taken up by cancer cells, with sEVs showing an approximately three-fold higher efficiency compared to leaf nanovesicles. These results support the potential of plant-derived vesicles as vehicles for therapeutic delivery. Copyright © 2020. Published by Elsevier Inc.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.nano.2020.102271
  3. PMID: 32702466
  4. View record in Web of Science®
  5. WOS: 000573096700017
  6. PII : S1549-9634(20)30125-8

Library Notes

  1. Fiscal Year: FY2019-2020
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