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RNA-Seq analysis on ets1 mutant embryos of Xenopus tropicalis identifies microseminoprotein beta gene 3 as an essential regulator of neural crest migration

  1. Author:
    Wang, Chengdong
    Qi, Xufeng
    Zhou, Xiang
    Sun, Jianmin
    Cai, Dongqing
    Lu, Gang
    Chen,Jack
    Jiang, Zhihua
    Yao, Yong-Gang
    Chan, Wai Yee
    Zhao, Hui

  2. Author Address

    Key Laboratory for Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, China., Key Laboratory of Regenerative Medicine of Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou, China., Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China., Department of Pathogen Biology and Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, China., Advanced Biomedical Computing Center, National Cancer Institute, National Institutes of Health, Frederick, MD, USA., Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA, USA., Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China., Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, China., Kunming Institute of Zoology - The Chinese University of Hong Kong (KIZ-CUHK) Joint Laboratory of Bioresources and Molecular Research of Common Diseases., Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics, The Chinese University of Hong Kong, Hong Kong SAR, China.,
    1. Year: 2020
    2. Date: Jul 26
    3. Epub Date: 2020 07 26
  2. Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  4. Type of Article: Article
  5. ISSN: 0892-6638
  1. Abstract:

    The proto-oncogene ets1 is highly expressed in the pre-migratory and migratory neural crest (NC), and has been implicated in the delamination and migration of the NC cells. To identify the downstream target genes of Ets1 in this process, we did RNA sequencing (RNA-Seq) on wild-type and ets1 mutant X. tropicalis embryos. A list of genes with significantly differential expression was obtained by analyzing the RNA-Seq data. We validated the RNA-Seq data by quantitative PCR, and examined the expression pattern of the genes identified from this assay with whole mount in situ hybridization. A majority of the identified genes showed expression in migrating NC. Among them, the expression of microseminoprotein beta gene 3 (msmb3) was positively regulated by Ets1 in both X. laevis and X. tropicalis. Knockdown of msmb3 with antisense morpholino oligonucleotides or disruption of msmb3 by CRISPR/Cas9 both impaired the migratory streams of NC. Our study identified msmb3 as an Ets1 target gene and uncovered its function in maintaining neural crest migration pattern. © 2020 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.

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External Sources

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  2. DOI: 10.1096/fj.202000603R
  3. PMID: 32713114
  4. View record in Web of Science®
  5. WOS: 000556409300001

Library Notes

  1. Fiscal Year: FY2019-2020
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