Skip NavigationSkip to Content
Return Return to Search Results

A new genre of fluorescence recovery assay to evaluate polo-like kinase 1 ATP-competitive inhibitors

  1. Author:
    Tsuji, Kohei
    Hymel, David
    Burke,Terrence

  2. Author Address

    Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland, 21702 USA. burkete@mail.nih.gov.,
    1. Year: 2020
    2. Date: Sep 28
    3. Epub Date: 2020 09 03
  2. Journal: Analytical methods : advancing methods and applications
    1. 12
    2. 36
    3. Pages: 4418-4421
  4. Type of Article: Article
  5. ISSN: 1759-9660
  1. Abstract:

    Using a probe consisting of a fluorescein-labeled variant of the potent polo-like kinase 1 (Plk1) inhibitor BI2536 [FITC-PEG-Lys(BI2536) 4], we were able to determine half maximal inhibitory concentration (IC50) of ATP-competitive Type 1 inhibitors of Plk1 by means of a fluorescence recovery assay. This methodology represents a cost-effective and simple alternative to traditional kinase assays for initial screening of potential Plk1 inhibitors.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1039/d0ay01223h
  3. PMID: 32970049
  4. View record in Web of Science®
  5. WOS: 000572249700012

Library Notes

  1. Fiscal Year: FY2020-2021
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel