Skip NavigationSkip to Content
Return Return to Search Results

Foot Fat Pad: Characterization by Mesenchymal Stromal Cells in Rats

  1. Author:
    Zhang, Zijun [ORCID]
    Paudel Kattel,Sharada
    Feltham, Tyler
    Lobao, Mario H
    Schon, Lew

  2. Author Address

    Center for Orthopaedic Innovation, Mercy Medical Center, Baltimore, MD, USA., Frederick National Laboratory for Cancer Research, Frederick, MD, USA., Philadelphia College of Osteopathic Medicine-GA, Suwanee, GA, USA., Columbia University Medical Center, New York, NY, USA., Institute for Foot and Ankle Reconstruction, Mercy Medical Center, Baltimore, MD, USA.,
    1. Year: 2020
    2. Date: NOV 3
    3. Epub Date: 2020 10 25
  2. Journal: Anatomical record (Hoboken, N.J. : 2007)
  4. Type of Article: Article
  5. ISSN: 1932-8486
  1. Abstract:

    Foot fat pad (FFP) is a highly functionalized fat depot in the foot, of great significance in weight-bearing. Mesenchymal stromal cells (MSCs) in subcutaneous adipose tissues are widely studied for regenerative potentials. MSCs in FFP, which may contribute to the physiological and pathological conditions of the foot, have not been characterized. In this study, MSCs were isolated from FFP (designated as MSCs-ffp) and subcutaneous adipose tissue (designated as MSCs-sub) from rats. The cell surface markers, proliferation and efficiency of colony formation were compared between MSCs-ffp and MSCs-sub. In addition, MSCs-ffp were induced for osteogenic, chondrogenic and adipogenic differentiation. The tri-lineage differentiation potentials were compared between MSCs-ffp and MSCs-sub by the expression of Runx2, Sox9 and PPAR-?, respectively, using quantitative polymerized chain reaction (qPCR). The expression of elastin and associated genes by MSCs-ffp were also evaluated. MSCs-ffp, like MSCs-sub, expressed CD44, CD73 and CD90. MSCs-ffp and MSCs-sub proliferated at similar rates but MSCs-ffp formed more colonies than MSCs-sub. MSCs-ffp were capable of differentiating into osteogenic, chondrogenic and adipogenic lineages. Under the conditions of osteogenic and adipogenic differentiation, MSCs-sub expressed more Runx2 and PPAR-?, respectively, than MSCs-ffp. The undifferentiated MSCs-ffp up-regulated the expression of fibulin-5. In conclusion, MSCs-ffp shared common biology with MSCs-sub but were more efficient in colony formation, less adipogenic and osteogenic, and participated in elastogenesis. The unique features of MSCs-ffp may relate to their roles in the physiological functions of FFP. This article is protected by copyright. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1002/ar.24549
  3. PMID: 33099882
  4. View record in Web of Science®
  5. WOS: 000583983600001

Library Notes

  1. Fiscal Year: FY2020-2021
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel