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Expression of functional formyl peptide receptors by human astrocytoma cell lines

  1. Author:
    Le, Y. Y.
    Hu, J. Y.
    Gong, W. H.
    Shen, W. P.
    Li, B. Q.
    Dunlop, N. M.
    Halverson, D. O.
    Blair, D. G.
    Wang, J. M.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Mol Immunoregulat Lab, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Mol Immunoregulat Lab, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Intramural Res Support Program, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, Basic Res Lab, Frederick, MD 21702 USA.
    1. Year: 2000
  2. Journal: Journal of Neuroimmunology
    1. 111
    2. 1-2
    3. Pages: 102-108
  4. Type of Article: Article
  1. Abstract:

    Activation of astrocytes is important in the pathogenesis of a variety of diseases in the central nervous system, such as infection and neurodegeneration. We found that the bacterial chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (fMLF) induced potent migration and Ca2+ mobilization in human astrocytoma cell lines. The effect of fMLF was pertussis toxin- sensitive, suggesting the involvement of seven transmembrane, G protein-coupled receptor(s) for fMLF. Scatchard analyses revealed that astrocytoma cell Lines express both high- and low-affinity binding sites for [H-3]fMLF. RT-PCR confirmed the expression of transcripts of fMLF receptors, the high-affinity FPR and the low-affinity FPRL1 by these cells. Both fMLF and F peptide, a synthetic peptide domain of HIV-1 envelope protein which specifically activates FPRL1, increased secretion of IL-6 by astrocytoma cells. Our study demonstrates for the first time that FPR and FPRL1 expressed by astrocytoma cell lines are functional, and suggests a molecular basis for the involvement of these receptors in host defense in the brain. (C) 2000 Elsevier Science B.V. All rights reserved.

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