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Newcastle Disease Virus-Like Particles Displaying Prefusion-Stabilized SARS-CoV-2 Spikes Elicit Potent Neutralizing Responses

  1. Author:
    Yang, Yongping
    Shi, Wei
    Abiona, Olubukola M.
    Nazzari, Alexandra
    Olia, Adam S.
    Ou, Li
    Phung, Emily
    Stephens, Tyler
    Tsybovsky,Yaroslav
    Verardi, Raffaello
    Wang, Shuishu
    Werner, Anne
    Yap, Christina
    Ambrozak, David
    Bylund, Tatsiana
    Liu, Tracy
    Nguyen, Richard
    Wang, Lingshu
    Zhang, Baoshan
    Zhou, Tongqing
    Chuang, Gwo-Yu
    Graham, Barney S.
    Mascola, John R.
    Corbett, Kizzmekia S.
    Kwong, Peter D.

  2. Author Address

    NIAID, Vaccine Res Ctr, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA.Leidos Biomed Res Inc, Electron Microscopy Lab, Canc Res Technol Program, Frederick Natl Lab Canc Res, Frederick, MD 21702 USA.
    1. Year: 2021
    2. Date: Jan 21
  2. Journal: Vaccines
  3. MDPI,
    1. 9
    2. 2
  5. Type of Article: Article
  6. Article Number: ARTN 73
  7. ISSN: 2076-393X
  1. Abstract:

    The COVID-19 pandemic highlights an urgent need for vaccines that confer protection from SARS-CoV-2 infection. One approach to an effective COVID-19 vaccine may be through the display of SARS-CoV-2 spikes on the surface of virus-like particles, in a manner structurally mimicking spikes on a native virus. Here we report the development of Newcastle disease virus-like particles (NDVLPs) displaying the prefusion-stabilized SARS-CoV-2 spike ectodomain (S2P). Immunoassays with SARS-CoV-2-neutralizing antibodies revealed the antigenicity of S2P-NDVLP to be generally similar to that of soluble S2P, and negative-stain electron microscopy showed S2P on the NDVLP surface to be displayed with a morphology corresponding to its prefusion conformation. Mice immunized with S2P-NDVLP showed substantial neutralization titers (geometric mean ID50 = 386) two weeks after prime immunization, significantly higher than those elicited by a molar equivalent amount of soluble S2P (geometric mean ID50 = 17). Neutralizing titers at Week 5, two weeks after a boost immunization with S2P-NDVLP doses ranging from 2.0 to 250 mu g, extended from 2125 to 4552, and these generally showed a higher ratio of neutralization versus ELISA than observed with soluble S2P. Overall, S2P-NDVLP appears to be a promising COVID-19 vaccine candidate capable of eliciting substantial neutralizing activity.

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External Sources

  1. View Full Text
  2. DOI: 10.3390/vaccines9020073
  3. PMID: 33494381
  4. PMCID: PMC7912142
  5. View record in Web of Science®
  6. WOS: 000623283900001

Library Notes

  1. Fiscal Year: FY2020-2021
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