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Relative domain orientation of the L289K HIV-1 reverse transcriptase monomer

  1. Author:
    Xi, Zhaoyong
    Ilina, Tatiana V [ORCID]
    Guerrero, Michel
    Fan,Lixin
    Sluis-Cremer, Nicolas
    Wang,Yun-Xing
    Ishima, Rieko [ORCID]

  2. Author Address

    Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Basic Science Program, Frederick National Laboratory for Cancer Research, SAXS Core Facility of the National Cancer Institute, Frederick, Maryland, USA., Department of Medicine, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA., Protein-Nucleic Acid Interaction Section, Structural Biophysics Laboratory, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.,
    1. Year: 2022
    2. Date: May
  2. Journal: Protein Science : a publication of the Protein Society
    1. 31
    2. 5
    3. Pages: e4307
  4. Type of Article: Article
  5. Article Number: e4307
  1. Abstract:

    HIV-1 reverse transcriptase (RT) is a heterodimer comprised p66 and p51 subunits (p66/p51). Several single amino acid substitutions in RT, including L289K, decrease p66/p51 dimer affinity, and reduce enzymatic functioning. Here, small-angle X-ray scattering (SAXS) with proton paramagnetic relaxation enhancement (PRE), 19 F site-specific NMR, and size exclusion chromatography (SEC) were performed for the p66 monomer with the L289K mutation, p66L289K . NMR and SAXS experiments clearly elucidated that the thumb and RNH domains in the monomer do not rigidly interact with each other but are spatially close to the RNH domain. Based on this structural model of the monomer, p66L289K and p51 were predicted to form a heterodimer while p66 and p51L289K not. We tested this hypothesis by SEC analysis of p66 and p51 containing L289K in different combinations and clearly demonstrated that L289K substitution in the p51 subunit, but not in the p66 subunit, reduces p66/p51 formation. Based on the derived monomer model and the importance of the inter-subunit RNH-thumb domain interaction in p66/p51, validated by SEC, the mechanism of p66 homodimer formation was discussed. © 2022 The Protein Society.

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  2. DOI: 10.1002/pro.4307
  3. PMID: 35481647

Library Notes

  1. Fiscal Year: FY2021-2022
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