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A gender-specific mRNA encoding a cytotoxic ribonuclease contains a 3 ' UTR of unusual length and structure

  1. Author:
    Chen, S. L.
    Le, S. Y.
    Newton, D. L.
    Maizel, J. V.
    Rybak, S. M.

  2. Author Address

    Maizel JV NCI, Lab Expt & Computat Biol, Div Basic Sci, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA NCI, Lab Expt & Computat Biol, Div Basic Sci, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA NCI, Lab Biochem Physiol, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Nucleic Acids Research
    1. 28
    2. 12
    3. Pages: 2375-2382
  4. Type of Article: Article
  1. Abstract:

    A cDNA (2855 nt) encoding a putative cytotoxic ribonuclease (rapLR1) related to the antitumor protein onconase was cloned from a library derived from the liver of gravid female amphibian Rana pipiens. The cDNA was mainly comprised (83%) of 3' untranslated region (UTR). Secondary structure analysis predicted two unusual folding regions (UFRs) in the RNA 3' UTR. Two of these regions (711-1442 and 1877-2130 nt) contained remarkable, stalk-like, stem-loop structures greater than 38 and 12 standard deviations more stable than by chance, respectively, Secondary structure modeling demonstrated similar structures in the 3' UTRs of other species at low frequencies (0.01-0.3%). The size of the rapLR1 cDNA corresponded to the major hybridizing RNA cross-reactive with a genomic clone encoding onconase (3.6 kb). The transcript was found only in liver mRNA from female frogs. In contrast, immunoreactive onconase protein was detected only in oocytes. Deletion of the 3' UTR facilitated the in vitro translation of the rapLR1 cDNA. Taken together these results suggest that these unusual UFRs may affect mRNA metabolism and/or translation. [References: 50]

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