Skip NavigationSkip to Content
Return Return to Search Results

cDNA cloning and mapping of mouse pleckstrin (Plek), a gene upregulated in transformation-resistant cells

  1. Author:
    Cmarik, J. L.
    Hegamyer, G.
    Gerrard, B.
    Dean, M.
    Colburn, N. H.

  2. Author Address

    Colburn NH NCI, Gene Regulat Sect, BRL Bldg 560,Room 21-89 Frederick, MD 21701 USA NCI, Gene Regulat Sect, BRL Frederick, MD 21701 USA NCI, Intramural Res Support Program SAIC Frederick Frederick, MD 21701 USA NCI, Lab Genom Divers, Frederick Canc Res & Dev Ctr Frederick, MD 21701 USA
    1. Year: 2000
  2. Journal: Genomics
    1. 66
    2. 2
    3. Pages: 204-212
  4. Type of Article: Article
  1. Abstract:

    Changes that occur during tumor promotion, the rate-limiting phase of multistep carcinogenesis, may offer the best targets for prevention of cancer or reversal of early disease. The murine epidermal JB6 promotion-sensitive (P+) and -resistant (P-) cell lines provide a cell culture model for tumor promoter-induced neoplastic transformation ideally suited to the identification of molecular events that mediate or inhibit transformation. A differential display comparison of P+ and P- cell mRNAs yielded seven differentially expressed sequences. One of the sequences preferentially expressed in P- cells identified an similar to 3.6-kb message that was induced to higher levels in P- cells following exposure to the tumor promoter 12-O-tetradecanoylphorbol acetate than in P+ cells. The message was detected in mRNA from heart, lung, and spleen. cDNA cloning of the P- preferential sequence revealed a high degree of identity to human pleckstrin (PLEK), the major PKC substrate in platelets (Tyers et al., 1988, Nature 333: 470). We report the complete mouse cDNA sequence of pleckstrin and the localization of the gene to chromosome 11, its expression in a nonhematopoetic cell line, and its potential role in blocking neoplastic transformation. [References: 54]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel