Skip NavigationSkip to Content
Return Return to Search Results

Characterization of the Ly491 promoter

  1. Author:
    Gosselin, P.
    Makrigiannis, A. P.
    Nalewaik, R.
    Anderson, S. K.

  2. Author Address

    Anderson SK NCI, Frederick Canc Res & Dev Ctr, Intramural Res Support Program, SAIC Frederick Bldg 560,Room 31-93 Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Intramural Res Support Program, SAIC Frederick Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Expt Immunol Lab, Div Basic Sci Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Immunogenetics
    1. 51
    2. 4-5
    3. Pages: 326-331
  4. Type of Article: Article
  1. Abstract:

    Fourteen potential Ly49 genes have been identified in the C57Bl/6 mouse strain. and cDNAs containing a complete coding region have been isolated for 10 members of this gene family. Ly49 proteins are primarily expressed in natural killer (NK) cells. Although the sequence of the Ly49a promoter region has been published, no study of the cell-specific activity of the promoter has been reported. A 12-kb genomic fragment of the Ly49I gene was isolated and characterized by DNA sequencing. Approximately 5 kb of DNA sequence upstream of the first Ly49I exon was determined and this region was used to perform promoter analysis using luciferase reporter plasmid constructs. A core promoter was identified that was preferentially transcribed in a Ly49-expressing cell line, EL-4. Electrophoretic mobility shift assays using oligonucleotide probes from the core Ly49i promoter and comparable regions from the Ly49a promoter demonstrated the importance of TATA-related elements in generating EL-4 and NK cell-specific DNA/protein complexes. [References: 29]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel