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Integration and viral oncogene expression of human papillomavirus type 16 in oropharyngeal squamous cell carcinoma and gastric cancer

  1. Author:
    Xu, Qiang
    Dong, Haoru
    Wang, Zhiyu
    Zhang, Pei
    Albers, Andreas E
    Kaufmann, Andreas M
    Zheng,Zhi-Ming
    Qian, Xu [ORCID]

  2. Author Address

    Zhejiang Cancer Research Institute, Zhejiang Cancer Hospital, Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China., Department of Clinical Laboratory, Zhejiang Cancer Hospital, Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, China., Postgraduate Education Base, Wenzhou Medical University, Wenzhou, China., Department of Clinical Medicine, Oto-Rhino-Laryngology, Medical School Berlin, Berlin, Germany., Clinic for Gynecology, Berlin Institute of Health, Charit 233;-Universit 228;tsmedizin Berlin, Freie Universit 228;t Berlin, Humboldt-Universit 228;t zu Berlin, Berlin, Germany., Tumor Virus RNA Biology Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland, USA.,
    1. Year: 2023
    2. Date: May
  2. Journal: Journal of Medical Virology
    1. 95
    2. 5
    3. Pages: e28761
  4. Type of Article: Article
  5. Article Number: e28761
  1. Abstract:

    Persistent high-risk human papillomavirus (HR-HPV) infections cause cervical cancer and a fraction of head and neck cancer. To investigate whether HR-HPV infection might be also involved in the development of gastric cancer (GC), we developed a platform utilizing a rolling circle amplification (RCA)-based nested L1 polymerase chain reaction with Sanger sequencing to genotype the HPV DNA in cancer tissues of 361 GC and 89 oropharyngeal squamous cell carcinomas (OPSCC). HPV transcriptional activity was determined by E6/E7 mRNA expression and a 3' rapid amplification of cDNA ends was performed to identify HPV integration and expression of virus-host fusion transcripts. Ten of 361 GC, 2 of 89 OPSCC, and 1 of 22 normal adjacent tissues were HPV L1 DNA-positive. Five of the 10 HPV-positive GC were genotyped as HPV16 by sequencing and 1 of 2 GC with RCA/nested HPV16 E6/E7 DNA detection exhibited HPV16 E6/E7 mRNA. Two OPSCC displayed HPV16 L1 DNA and E6/E7 mRNA, of which 1 OPSCC tissue showed virus-host RNA fusion transcripts from an intron region of KIAA0825 gene. Together, our data reveal viral oncogene expression and/or integration in GC and OPSCC and a possible etiology role of HPV infections in gastric carcinogenesis. © 2023 The Authors. Journal of Medical Virology published by Wiley Periodicals LLC.

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External Sources

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  2. DOI: 10.1002/jmv.28761
  3. PMID: 37212316

Library Notes

  1. Fiscal Year: FY2022-2023
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