Skip NavigationSkip to Content
Return Return to Search Results

Evi9 encodes a novel zinc finger protein that physically interacts with BCL6, a known human B-Cell proto-oncogene product

  1. Author:
    Nakamura, T.
    Yamazaki, Y.
    Saiki, Y.
    Moriyama, M.
    Largaespada, D. A.
    Jenkins, N. A.
    Copeland, N. G.

  2. Author Address

    Nakamura T Japanese Fdn Canc Res, Inst Canc, Toshima Ku 1-37-1 Kami Ikebukuor Tokyo 1708455 Japan Japanese Fdn Canc Res, Inst Canc, Toshima Ku Tokyo 1708455 Japan Japan Sci & Technol Corp, PRESTO, Toshima Ku Tokyo 1708455 Japan Tottori Univ, Sch Med, Dept Mol Biol Tottori 6830826 Japan Univ Minnesota, Ctr Canc, Dept Genet Cell Biol & Dev Minneapolis, MN 55455 USA NCI, Mammalian Genet Lab, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Molecular and Cellular Biology
    1. 20
    2. 9
    3. Pages: 3178-3186
  4. Type of Article: Article
  1. Abstract:

    Evi9 is a common site of retroviral integration in BXH2 murine myeloid leukemias. Here we show that Evi9 encodes a novel zinc finger protein with three tissue-specific isoform;: Evi9a (773 amino acids [aa]) contains two C2H2-type zinc finger motifs, a proline-rich region, and an acidic domain; Evi9b (486 aa) lacks the first zinc finger motif and part of the proline-rich region; Evi9c (239 aa) lacks all but the first zinc linger motif, Proviral integration sites are located in the first intron of the gene and lead to increased gene expression. Evi9a and Evi9c, but not Evi9b? show transforming activity for NIH 3T3 cells, suggesting that Evi9 is a dominantly acting proto-oncogene, Immunolocalization studies show that Evi9c is restricted to the cytoplasm whereas Evi9a and Evi9b are located in the nucleus, where they form a speckled localization pattern identical to that observed for BCL6, a human B-cell proto-oncogene product, Coimmunoprecipitation and glutathione S-transferase pull-down experiments show that Evi9a and Evi9b, but not Evi9c, physically interact with BCL6, while deletion mutagenesis localized the interaction domains in or near the second zinc finger and POZ domains of Evi9 and BCL6, respectively, These results suggest that Evi9 is a leukemia disease gene that functions, in part, through its interaction with BCL6. [References: 41]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel