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Nanoparticles may influence mast cells gene expression profiles without affecting their degranulation function

  1. Author:
    Newton,Hannah
    Cedrone,Edward
    Grunberger, Jason
    Xie,Shaojun
    Zhao,Yongmei
    Tran,Bao
    Toms, Bradley
    Xu, Weining
    Plant-Hately, Alexander
    Liptrott, Neill J
    Dobrovolskaia,Marina

  2. Author Address

    Nanotechnology Characterization Lab., Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD 21702, USA., Nanotechnology Characterization Lab., Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD 21702, USA; Utah Center for Nanomedicine, Department of Molecular Pharmaceutics, University of Utah, Salt Lake City, UT, USA., Sequencing Facility Bioinformatics Group, Biomedical Informatics and Data Science Directorate, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD 21702, USA., CCR Sequencing Facility, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD 21702, USA., 10x Genomics, Inc., Pleasanton, CA 94588-3260, USA., Immunocompatibility Group, Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L7 3NY, UK; Centre of Excellence for Long-Acting Therapeutics (CELT), Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L7 8TX, UK., Nanotechnology Characterization Lab., Cancer Research Technology Program, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute, Frederick, MD 21702, USA. Electronic address: marina@mail.nih.gov.,
    1. Year: 2025
    2. Date: Apr 02
    3. Epub Date: 2025 04 02
  2. Journal: Nanomedicine : nanotechnology, biology, and medicine
    1. Pages: 102818
  4. Type of Article: Article
  5. Article Number: 102818
  1. Abstract:

    An in vitro method for monitoring nanoparticle effects on IgE-dependent mast cell degranulation was developed and validated. The assayed nanoparticles included four clinical-grade nanomedicines (Abraxane, Doxil, AmBisome, and Feraheme) and three commercial research-grade nanomaterials (generation 5 PAMAM dendrimers with carboxy-, hydroxy-, or amine- surface functionalities). Most of the tested materials did not alter IgE-dependent mast cell degranulation, suggesting that nanoparticles and nanomedicines are unlikely to worsen pre-existing allergies to other antigens. Two clinical-grade formulations containing cytotoxic oncology drugs-Abraxane and Doxil-decreased degranulation. Abraxane but not Doxil decreased FceR expression on the cell surface. Single-cell sequencing revealed the most differentially expressed genes (DEG) in Abraxane and Doxil-treated cultures. Interestingly, Feraheme induced DEG without affecting degranulation. These data demonstrate that some nanomaterials have more effects on immune cells than can be detected by a functional immunoassay. Copyright © 2025. Published by Elsevier Inc.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.nano.2025.102818
  3. PMID: 40185352
  4. PII : S1549-9634(25)00018-8

Library Notes

  1. Fiscal Year: FY2024-2025
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