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Cloning and Expression Analysis of Murine Phospholipase D1

  1. Author:
    Colley, W. C.
    Altshuller, Y. M.
    Sueling, C. K.
    Copeland, N. G.
    Gilbert, D. J.
    Jenkins, N. A.
    Branch, K. D.
    Tsirka, S. E.
    Bollag, R. J.
    Bollag, W. B.
    Frohman, M. A.

  2. Author Address

    Frohman MA SUNY STONY BROOK DEPT PHARMACOL SCI GENET PROGRAM STONY BROOK, NY 11794 USA SUNY STONY BROOK DEPT PHARMACOL SCI GENET PROGRAM STONY BROOK, NY 11794 USA MED COLL GEORGIA INST MOL & MED GENET AUGUSTA, GA 30912 USA NCI FREDERICK CANC RES & DEV CTR ABL BASIC RES PROGRAM MAMMALIAN GENET LAB FREDERICK, MD 21702 USA SUNY STONY BROOK INST CELL & DEV BIOL STONY BROOK, NY 11794 USA
    1. Year: 1997
  2. Journal: Biochemical Journal
    1. 326
    2. Part 3
    3. Pages: 745-753
  4. Type of Article: Article
  1. Abstract:

    Activation of phosphatidylcholine-specific phospholipase D (PLD) occurs as part of the complex signal-transduction cascade initiated by agonist stimulation of tyrosine kinase and G-protein-coupled receptors. A variety of mammalian PLD activities have been described, and cDNAs for two PLDs recently reported (human PLD 1 and murine PLD2). We describe here the cloning and chromosomal localization of murine PLD1. Northern-blot hybridization and RNase protection analyses were used to examine the expression of murine PLD1 and PLD2 in a variety of cell lines and tissues. PLD1 and PLD2 were expressed in all RNA samples examined, although the absolute expression of each isoform varied, as well as the ratio of PLD1 to PLD2. Moreover, in situ hybridization of adult brain and murine embryo sections revealed high levels of expression of individual PLDs in some cell types and no detectable expression in others. Thus the two PLDs probably carry out distinct roles in restricted subsets of cells rather than ubiquitous roles in all cells. [References: 41]

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