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Potential cellular signatures of viral infections in human hematopoietic cells

  1. Author:
    Mikovits, J.
    Ruscetti, F.
    Zhu, W.
    Bagni, R.
    Dorjsuren, D.
    Shoemaker, R.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Lab Leukocyte Biol, DBS, POB B, Bldg 439, Frederick, MD 21702 USA. Lab Antiviral Drug Mech, Frederick, MD USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Lab Leukocyte Biol, DBS, Frederick, MD 21702 USA. SAIC Frederick, Intramural Res Support Program, Frederick, MD USA. DCTD, Dev Therapeut Program, Screening Technol Branch, Frederick, MD USA. Mikovits J NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Lab Leukocyte Biol, DBS, POB B, Bldg 439, Frederick, MD 21702 USA.
    1. Year: 2001
  2. Journal: Disease Markers
    1. 17
    2. 3
    3. Pages: 173-178
  4. Type of Article: Article
  1. Abstract:

    Expression profiling of cellular genes was performed using a 10,000 cDNA human gene array in order to identify expression changes following chronic infection of human hematopoietic cells with Kapsosi's Sarcoma -associated Virus (KSHV) also known as Human Herpesvirus 8 (HHV8) and Human T cell leukemia virus-1 (HTLV-1). We performed cell-free in vitro infection of primary bone marrow derived CD34+ cells using semi-purified HHV8 and a mature IL-2 dependent T cell line, KIT 225, using highly concentrated viral stocks prepared from an infectious molecular clone of HTLV-1. Thirty days post infection, mRNA was isolated from infected cultures and uninfected controls and submitted for microarray analysis. More than 400 genes were differentially expressed more than two-fold following HHV8 infection of primary bone marrow derived CD34+ cells. Of these 400, interferon regulatory factor 4 (IRF4), cyclin B2, TBP- associated factor, eukaryotic elongation factor and pim 2 were up-regulated more than 3.5 fold. In contrast, less than 100 genes were differentially expressed more than twofold following chronic infection of a mature T cell line with HTLV-1. Of these, only cdc7 was up-regulated more than 3.5 fold. These data may provide insight into cellular si.-natures of infection useful for diagnosis of infection as well as potential targets for therapeutic intervention.

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