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The separation of a penicillium fungal extract by thin layer chromatography and HPLC: A comparative study

  1. Author:
    Xu, H. Y.
    Issaq, S. H.
    McCloud, T. G.
    Issaq, H. J.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, POB B, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Frederick, MD 21702 USA.
    1. Year: 2001
  2. Journal: Journal of Liquid Chromatography & Related Technologies
    1. 24
    2. 5
    3. Pages: 625-633
  4. Type of Article: Article
  1. Abstract:

    The separation of a Penicillium fungal extract by two dimensional-multimodal thin layer chromatography and gradient high performance liquid chromatography is reported. The fungal extract was resolved on a cyano derivatized silica gel TLC plate using two dimensional development; first dimension in methylene chloride: hexane:acetic acid (9:10:1), taken out, dried, turned 90 degrees, and developed in the second dimension in acetonitrile:methanol: water (40:37:32). Over 20 different spots were observed under UV radiation. Three different mobile phase gradients, acetonitrile, methanol, and tetrahydrofuran as primary organic solvents were used. Different selectivities; resulted in each gradient. The best result was obtained using the acetonitrile gradient, 25% to 100% acetonitrile in 30 minutes. Also, it was found that using photo diode array detection in natural products research is a must when quantitation is required, since the mixture contains compounds of different absorptivities. The results show that changing the wave length of detection resulted in different peak heights (areas).

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