Skip NavigationSkip to Content
Return Return to Search Results

Differential zinc and DNA binding by partial peptides of human protamine HP2

  1. Author:
    Bal, W.
    Dyba, M.
    Szewczuk, Z.
    Jezowska-Bojczuk, M.
    Lukszo, J.
    Ramakrishna, G.
    Kasprzak, K. S.

  2. Author Address

    Univ Wroclaw, Fac Chem, F Joliot Curie 14, PL-50383 Wroclaw, Poland. Univ Wroclaw, Fac Chem, PL-50383 Wroclaw, Poland. NCI, Comparat Carcinogenesis Lab, Frederick, MD 21701 USA. NIAID, Peptide Anal & Synth Unit, Res Technol Branch, Rockville, MD USA. Bal W Univ Wroclaw, Fac Chem, F Joliot Curie 14, PL-50383 Wroclaw, Poland.
    1. Year: 2001
  2. Journal: Molecular and Cellular Biochemistry
    1. 222
    2. 1-2
    3. Pages: 97-106
  4. Type of Article: Article
  1. Abstract:

    The Zn(II) binding by partial peptides of human protamine HP2: HP2(1-15); HP2(1-25), HP2(26-40), HP2(37-47), and HP2(43)-57 was studied by circular dichroism (CD). Precipitation of a 20- mer DNA by these partial peptides and the effects of Zn(II) thereon were investigated using polyacrylamide gel electrophoresis (GE). The results of this study suggest that reduced HP2 (thiol groups intact) can bind Zn(II) at various parts of the molecule. In the absence of DNA, the primary Zn(II) binding site in reduced HP2 is located in the 37-47 sequence (involving Cys-37, His-39, His-43, and Cys-47), while in the presence of DNA, the strongest Zn(II) binding is provided by sequences 12-22 (by His-12, Cys-13, His-19, and His-22) and 43-57 (His-43, Cys-47, Cys-53, and His-57). In its oxidized form, HP2 can bind zinc through His residues of the 7- 22 sequence. Zn(II) markedly enhances DNA binding by all partial peptides. These findings suggest that Zn(II) ions may be a regulatory factor for sperm chromatin condensation processes.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel