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Effects of different fixatives on beta-galactosidase activity

  1. Author:
    Ma, W. B.
    Rogers, K.
    Zbar, B.
    Schmidt, L.

  2. Author Address

    NCI Frederick, SAIC Frederick, IRSP, Bldg 560,Room 12-69, Ft Detrick, MD 21702 USA NCI Frederick, SAIC Frederick, IRSP, Ft Detrick, MD 21702 USA NCI Frederick, SAIC Frederick, Immunobiol Lab, Ft Detrick, MD 21702 USA NCI Frederick, SAIC Frederick, Pathol & Histol Lab, Ft Detrick, MD 21702 USA Schmidt L NCI Frederick, SAIC Frederick, IRSP, Bldg 560,Room 12-69, Ft Detrick, MD 21702 USA
    1. Year: 2002
  2. Journal: Journal of Histochemistry and Cytochemistry
    1. 50
    2. 10
    3. Pages: 1421-1424
  4. Type of Article: Article
  1. Abstract:

    beta-Galactosidase (beta-Gal) staining is widely used to demonstrate specific gene expression during evaluation of gene targets in vivo. This technique is extremely sensitive to fixation. Optimal fixation conditions are necessary to obtain the maximal P-Gal activity. In this experiment, Carnoy's and three different aldehyde fixatives were used at different temperatures and over different time points. Kidneys from LacZ- stop-human alkaline phosphatase (ZA/P) double reporter mice were used to generate positive material for the experiment. The results show that glutaraldehyde combinative solution (LacZ) produced the most consistent and reliable results. Paraformaldehyde and formaldehyde were effective as fixatives only at 4C for a period of less than 4 hr, and Carnoy's solution destroyed P-Gal activity.

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