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Microarray analysis of altered gene expression in the TM4 Sertoli-like cell line exposed to chromium(III) chloride

  1. Author:
    Cheng, R. Y. S.
    Alvord, W. G.
    Powell, D.
    Kasprzak, K. S.
    Anderson, L. M.

  2. Author Address

    NCI, Frederick Canc Res & Dev Ctr, Comparat Carcinogenesis Lab, Box B,Bldg 538,Ft Detrick, Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, Comparat Carcinogenesis Lab, Frederick, MD 21702 USA Data Management Serv Inc, Frederick, MD USA Cheng RYS NCI, Frederick Canc Res & Dev Ctr, Comparat Carcinogenesis Lab, Box B,Bldg 538,Ft Detrick, Frederick, MD 21702 USA
    1. Year: 2002
  2. Journal: Reproductive Toxicology
    1. 16
    2. 3
    3. Pages: 223-236
  4. Type of Article: Article
  1. Abstract:

    Chromium(III) chloride is a common human exposure metal that is a preconceptional carcinogen in mice, although it enters cells poorly, and is non-toxic and non-carcinogenic in most biologic systems. An indirect effect on sperm is postulated, and this effect might be mediated through the testicular Sertoli cells that influence spermatogenesis. To test this possibility, we exposed mouse TM4 Sertoli-like cultured cells to 1 mM CrCl3.6H(2)O, a non-toxic dose, for 7 days and then extracted mRNA for microarray analysis. The chromium(III) chloride had modest effects on the expression of many genes, in the range of 1.5-2.3-fold. These effects provided an opportunity for development of statistical approaches for sifting microarray data in a situation where differences were small. Data were winnowed by screening for those ratios that fell outside the 99% confidence limits and/or represented a greater than or equal to50% change in expression in the three comparison pairs. Fifty-two genes/clones were significant after the Bonferroni adjustment for multiple comparisons. The largest average increase was observed for the transcription factor Bach2, and this increase was confirmed by RT-PCR. The results show that Cr(III) has significant effects on gene expression in a Sertoli-like cell line. (C) 2002 Elsevier Science Inc. All rights reserved.

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