Skip NavigationSkip to Content
Return Return to Search Results

IL-7 withdrawal induces a stress pathway activating p38 and Jun N-terminal kinases

  1. Author:
    Rajnavolgyi, E.
    Benbernou, N.
    Rethi, B.
    Reynolds, D.
    Young, H. A.
    Magocsi, M.
    Muegge, K.
    Durum, S. K.

  2. Author Address

    NCI, Mol Immunoregulat Lab, Bldg 560,Room 31-71, Frederick, MD 21702 USA NCI, Mol Immunoregulat Lab, Frederick, MD 21702 USA Lorand Eotvos Univ, God, Hungary Sci Applicat Int Corp, Frederick, MD USA NCI, Expt Immunol Lab, Frederick, MD 21702 USA Natl Inst Haematol & Immunol, Dept Isotope & Membrane Diagnost, Budapest, Hungary Durum SK NCI, Mol Immunoregulat Lab, Bldg 560,Room 31-71, Frederick, MD 21702 USA
    1. Year: 2002
  2. Journal: Cellular Signalling
    1. 14
    2. 9
    3. Pages: 761-769
  4. Type of Article: Article
  1. Abstract:

    IL-7 delivers survival signals to cells at an early stage in lymphoid development. In the absence of IL-7, pro-T cells undergo programmed cell death, which has previously been associated with a decline in Bcl-2 and translocation of Bax from cytosol to mitochondria. A new, earlier feature of IL-7 withdrawal was identified using an IL-7-dependent thymocyte line. We observed that withdrawal of IL-7 induced increased expression of jun and fos family member genes including c-jun, junB, junD, c-fos and fra2. This transient response peaked 3-4 h after IL-7 was withdrawn and resulted in increased DNA- binding activity of AP-1 and in a change in the composition of the Jun/Fos family dimers, shown by electrophoretic mobility shift and supershift assays. Induction of jun and fos genes and the increased DNA-binding activity of AP-1 were attributable to the phosphorylation-induced activation of the stress kinases p38 and JNK and were blocked by the chemical kinase inhibitors SB203580 and SB202190. The stress response contributed to cell death following IL-7 withdrawal as shown by blocking the activity of the stress (MAP) kinases or by blocking the production of c-Jun and c-Fos using antisense oligonucleotides. (C) 2002 Elsevier Science Inc. All rights reserved.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel