Skip NavigationSkip to Content
Return Return to Search Results

Differential inducibility of the transcriptional repressor ICER and its role in modulation of Fas ligand expression in T and NK lymphocytes

  1. Author:
    Bodor, J.
    Bodorova, J.
    Bare, C.
    Hodge, D. L.
    Young, H. A.
    Gress, R. E.

  2. Author Address

    NCI, Expt Immunol Branch, Div Basic Sci, NIH, Bldg 10, Rm 4B14, 10 Ctr Dr, Bethesda, MD 20892 USA. NCI, Expt Immunol Branch, Div Basic Sci, NIH, Bethesda, MD 20892 USA. NCI, Transplantat Therapy Sect, Med Oncol Branch, NIH, Bethesda, MD 20892 USA. NCI, Frederick Canc Res & Dev Ctr, Biol Response Modifiers Program, Expt Immunol Lab, Frederick, MD 21701 USA. Gress RE NCI, Expt Immunol Branch, Div Basic Sci, NIH, Bldg 10, Rm 4B14, 10 Ctr Dr, Bethesda, MD 20892 USA.
    1. Year: 2002
  2. Journal: European Journal of Immunology
    1. 32
    2. 1
    3. Pages: 203-212
  4. Type of Article: Article
  1. Abstract:

    The engagement of antigen receptor can initiate apoptosis of T lymphocytes through the induced expression of Fas ligand (FasL). Forskolin, an activator of the cAMP/PKA pathway, results in antagonism of Fas-dependent, activation-induced cell death (AICD) by suppressed expression of the FasL. We report that forskolin-mediated induction of inducible cAMP early repressor (ICER) correlates with transcriptional attenuation of FasL expression in the AICD model 2134 T cell hybridoma. ICER is inducible in human peripheral blood CD3(+) T cells, but in CD19(+) B cells, its induction is less responsive to forskolin treatment. Increased expression of ICER correlates with decreased FasL expression in both T and NK cells. ICER binds specifically to the proximal DNA binding site of the nuclear factor of activated T cells (NFAT) in the FasL promoter and in the presence of the minimal NFAT DNA-binding domain, the proximal NFAT motif allows ICER and NFAT to form an NFAT/ICER ternary complex in vitro. Moreover, in the activated 2B4 T cell hybridoma, the proximal INFAT motif participates in the down- regulation of the FasL promoter mediated by ICER. These findings provide further insight into the mechanism involved in cAMP-mediated transcriptional attenuation of FasL expression in T and INK lymphocytes.

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel